FastQCFastQC Report
Mon 3 Jul 2023
SRR5278492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5278492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13903778
Total Bases695.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT2625961.8886665192726755No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2530161.819764383464696Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT2369601.7042849792337018No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT2160601.5539661234522013No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA1358750.9772523698235112No Hit
AAGAACCTTACCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC1056120.7595921051098486RNA PCR Primer, Index 6 (95% over 23bp)
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC841840.6054757203401838No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC837010.6020018443907836No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC675050.48551551959474615Illumina PCR Primer Index 6 (95% over 23bp)
AGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT646710.46513257044236467RNA PCR Primer, Index 6 (95% over 22bp)
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA565060.4064075246310751No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT530360.3814502791974958No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT501240.3605063314445901No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC492110.35393977090255613No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC474140.3410152262212472No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC436240.3137564480675684No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA414480.2981060255708916No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA377200.2712931693817321No Hit
TTTAAGGAGGTGTGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT365440.2628350366353663Illumina PCR Primer Index 6 (95% over 22bp)
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGCTGTAGGCACCATCAATAT362870.2609866181695364No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATAT354190.2547437106662664No Hit
AAGAACCTTACCAGGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCT320180.23028273322545859Illumina PCR Primer Index 6 (95% over 21bp)
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC310760.2235075962806656No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT307950.2214865628608282No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT294440.211769779408158Illumina PCR Primer Index 6 (95% over 21bp)
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT270940.19486789849492708No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC250930.18047612670455468No Hit
TGGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTAT232320.16709127547922586No Hit
TTACAGGAGGTGTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT229920.1653651259391512Illumina PCR Primer Index 6 (95% over 21bp)
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC229540.1650918189286394No Hit
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT228420.16428628247660454No Hit
TTTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTA226290.16275432475978832No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA215920.15529592028871575No Hit
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC209840.15092300812052667No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATAT199240.14329918098519698No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC197600.14211964546614597TruSeq Adapter, Index 6 (95% over 23bp)
TGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC195080.1403071884490676Illumina PCR Primer Index 6 (95% over 23bp)
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC195030.14027122700031602No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA190650.1371210040896798No Hit
GAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT175130.12595857039719707No Hit
CAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA173900.12507391875790883No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG170500.12262854024280306No Hit
ATAAGGAGGTGTCCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC170100.12234084865279063Illumina PCR Primer Index 6 (95% over 23bp)
TGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT165330.11891012644189226TruSeq Adapter, Index 6 (95% over 22bp)
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATAT164540.1183419355516177No Hit
AGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC159850.11496875165872182No Hit
GTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT154230.11092668481904701No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT152750.10986222593600099No Hit
AAAAAGGAGGTGCAAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT152050.10935876565347923RNA PCR Primer, Index 6 (95% over 22bp)
TTAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA151250.10878338247345433No Hit
TTTAAGGAGGTGGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT151100.10867549812719968TruSeq Adapter, Index 6 (95% over 22bp)
AGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT144350.10382070254573972Illumina PCR Primer Index 6 (100% over 21bp)
TTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCA143550.10324531936571485No Hit
TTTAGGAGGTGAAACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC141730.10193632263115825Illumina PCR Primer Index 6 (95% over 23bp)
ATTTGGAGGTGAAACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC141590.10183563057465388Illumina PCR Primer Index 6 (95% over 23bp)
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC139150.100080711875578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)