FastQCFastQC Report
Tue 4 Jul 2023
SRR5278493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5278493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74864264
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT13691521.8288458696394851No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12306261.6438096552982877Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT11506261.5369495918640168No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT9200601.2289708745416905No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA6181210.8256556158756867No Hit
AAGAACCTTACCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC4771400.6373401333378499RNA PCR Primer, Index 6 (95% over 23bp)
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC3781300.5050874473300105No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC3739260.4994719509965395No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC3148750.42059453092332544Illumina PCR Primer Index 6 (95% over 23bp)
AGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT3016490.4029278909360546RNA PCR Primer, Index 6 (95% over 22bp)
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA2693980.35984859211332126No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT2280020.3045538522892578No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT2271000.3033490050740364No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC2202450.2941924333885123No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC2141780.2860884333278158No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC2081270.2780058052798061No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA1899140.25367777608820147No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA1595780.21315644003392595No Hit
TTTAAGGAGGTGTGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT1554240.20760773124010143Illumina PCR Primer Index 6 (95% over 22bp)
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGCTGTAGGCACCATCAATAT1541630.2059233494902187No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1502770.200732621908899No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATAT1479480.1976216583121688No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT1474250.19692306064746729Illumina PCR Primer Index 6 (95% over 21bp)
AAGAACCTTACCAGGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCT1448620.19349953136519182Illumina PCR Primer Index 6 (95% over 21bp)
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC1411610.18855591768056384No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT1221280.16313257283875787No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC1128240.1507047474613522No Hit
TGGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTAT1066380.1424417930562972No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC1059550.1415294752647271No Hit
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT1057630.14127301111248486No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA995650.1329940276979147No Hit
TTACAGGAGGTGTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT987680.13192943431595078Illumina PCR Primer Index 6 (95% over 21bp)
TTTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTA984810.13154607383838035No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC965230.12893067378582657No Hit
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC950010.12689766107898956No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC897920.11993973519862562TruSeq Adapter, Index 6 (95% over 23bp)
TGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC883890.11806567683614708Illumina PCR Primer Index 6 (95% over 23bp)
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATAT866740.11577486422627489No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA859860.11485586768074019No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATAT833820.11137757261595466No Hit
GAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT805950.10765483515606325No Hit
CAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA801380.10704439704369496No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG786170.10501272008765089No Hit
ATAAGGAGGTGTCCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC770190.10287819032055133Illumina PCR Primer Index 6 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)