FastQCFastQC Report
Mon 3 Jul 2023
SRR5278494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5278494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28072475
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCA5767052.054343266847686No Hit
GGGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAAAAAA3696111.3166313265930418No Hit
GGGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAA3612371.286801395316943No Hit
GCGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCA2237690.7971117616099043No Hit
CCGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCA2118330.7545932448065231No Hit
GCGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAAAAAA2043910.7280832915515999No Hit
CCGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAAAAAA1750530.6235752280481147No Hit
GGGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAGATCG1637280.5832332204410192No Hit
GCGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAA1348430.4803388372418178No Hit
CCGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAA1249320.44503379199732124No Hit
GGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAA1122340.39980087256289304No Hit
GGCTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAAAAAA985600.35109123794749125No Hit
GCGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAGATCG929440.33108587682418456No Hit
CGCTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAAAAAA886990.315964303111856No Hit
CCGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAGATCG850730.303047736261231No Hit
CGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAA706690.25173768967645355No Hit
GGGTAACGGGGCTAAGTAGTAAACCGAAGCTGCGGCAATACAAAAAAAAA572340.20387942281540902No Hit
GGGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAACA523560.18650297132689583No Hit
GGGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAG470890.16774082085744133No Hit
GGGGGTTAAGTGACTAAGCGTACACGGTGGATGCCTTGGCAGTCAGAGGC425110.15143303182209622No Hit
GGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAG410260.14614315267891415No Hit
GGGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAACAAAAAA405900.14459002991364317No Hit
GGGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAGATCGG404000.14391321035997004No Hit
CCGTAACGGGGCTAAGTAGTAAACCGAAGCTGCGGCAATACAAAAAAAAA387350.13798213374488713No Hit
GGCGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCA379370.13513949161945998No Hit
GGCTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAGATCG368450.13124956028992812No Hit
GGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAA357480.1273418179195101No Hit
GGGGGGGCTGATTTAGGATTCGACGGAAAAAAAAAAAAAAAGATCGGAAG346090.1232844628056486No Hit
CGCTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAGATCG345440.12305291927412884No Hit
GCGTAACGGGGCTAAGTAGTAAACCGAAGCTGCGGCAATACAAAAAAAAA344740.12280356470172295No Hit
GGGATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAA327890.11680124392309549No Hit
GGGAGGCTGAGACACGACGTCGAGCACCAAAAAAAAAAAAAAAGATCGGA325010.11577532796805412No Hit
GGGGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCAAAAAA324180.11547966468934427No Hit
GGGTGAAGTCATTGATGCCATGCTTCCAGGAAAAAAAAAAAAAAAAATCG316570.11276882426647454No Hit
GGGACTCCGAATACCAGTAAGTACTATCCGGGAGACACACGGCGGGTGCA315420.11235917032609345No Hit
GGGGTGTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAAA307900.10968038977681877No Hit
GGGGGGCGATTAGCTCAGTTGGGAGAGCACCTGCAAAAAAAAAAAAAAAG304360.1084193680820804No Hit
GGCTAACGGGGCTAAGTAGTAAACCGAAGCTGCGGCAATACAAAAAAAAA287920.10256309783871925No Hit
GCCTGAAGTCATTGATGCCATGCTTCCAGGAAAAGCCTCTAAGCAAAAAA281550.10029397122982567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)