FastQCFastQC Report
Tue 4 Jul 2023
SRR5291505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5291505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77015703
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length35-84
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1032766613.409818514543717No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA34894044.530769523716482No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA23856303.0975890721921995No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC21625852.807979302610534No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC21400092.7786658001420825No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT17616742.2874218261696577No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC16508632.1435407789499763No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT16299582.1163969638763147No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT15601312.025731038253329No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT14944461.9404432366214979No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT12829581.6658394976930873No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12764881.6574386135253483No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA12702701.649364935356105No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT10089631.3100743883361032No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT7537960.9787562414381908No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT7351090.954492358525897No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATA6691450.8688422931100168No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATC6626310.860384277736191No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6310830.8194212029720744Illumina PCR Primer Index 6 (96% over 29bp)
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT6058950.7867161843604803No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAATA5930160.7699936206516221No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT5664010.7354357331517184No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT4818740.6256827909497885No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA4201460.5455329025562488No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATATCT4158530.5399587146532961No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC4008910.5205315077108366No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC3808140.49446279806080584No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA3630130.47134932988925654No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA3330530.43244817229026655No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT3263140.4236980087034978No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3003530.3899892986758817No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA2884130.3744859668423724No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAT2818350.3659448515324206No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA2722210.3534616830024911No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA2612630.33923341581391525No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATATCTC2596670.3371611111567728No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2466020.3201970382585484No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA2457820.3191323203269339No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA2444440.3173950122872994No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTCTGTAGGCACCATCAATATCT2400490.3116883838611458No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCT2283780.29653433144666613No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTCG2016370.2618128409475143No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTCGTA2001940.2599391970751731No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1912020.24826365604946826No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA1902710.24705481166613516No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCTC1785150.2317903921489881No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1649940.21423423220586585No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1568470.20365587002432478No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATA1488700.1932982420481184No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCAA1454140.18881084549731372No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATA1383290.17961142288086368No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1299510.16873312186736775No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATAT1289690.16745805722243423No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTC1288010.16723991989010345No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1276740.16577658195238446No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCCTGTAGGCACCATCAATAT1261300.16377179599334435No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA1227190.15934282908512826No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT1213200.15752631641887369No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA1208890.15696669028652507No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1125170.146096179892041No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1085490.14094398385222814No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATATC1078460.14003118299134398No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT1074950.13957543177915288No Hit
TGAACGCACGTTCGTGTGGAACCTGGCGCTCTGTAGGCACCATCAATATC1064470.13821467032508944No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1038480.13484003385647209No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCTCGT1026660.1333052819111448No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAT994910.12918274601739335No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA971150.12609766088871513No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC955710.12409287492967506No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT936840.12164272525045965No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT928060.1205026980017309No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTC890520.11562836737333942No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTCTGTAGGCACCATCAATATC877040.11387807496868528No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT865020.11231735429331859No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAT837960.10880378511899061No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC790530.10264530079015186No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC786390.1021077480783367No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT772910.10035745567368254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)