FastQCFastQC Report
Mon 3 Jul 2023
SRR5291506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5291506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80449451
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT899425011.180001713125426No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA23111492.8727964843414533No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC21586682.68326007594508No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC20942442.6031799769522355No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT18634112.3162507348869292No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATATCT18500302.299617930270276No Hit
TTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCGT16244972.01927667598378No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA15946401.9821639304909613No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA15742171.956777803244425No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT14042271.745477417863299No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT13529071.6816858078994226No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA11164971.387824262467621No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCTCGT10959601.362296431333012No Hit
TTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCG10056481.2500371195820839No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC7762340.964871718018312No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC6910820.8590263717275087No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC6310260.7843757690776535No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTCGTA6157180.76534767154595No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA6040830.7508851738466182No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTCG5678560.7058544128536067No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATATC5499550.6836031733765343No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC4929390.6127313410752797No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAATA4699100.5841059126680678No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCAA4555750.5662872702512289No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4353060.5410925675552466No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATATCT4253440.5287096365642073No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA3855410.4792338483453417No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT3802800.4726943382124509No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT3758990.46724868265415515No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA3675440.45686327927831355No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA3628520.4510310455692233No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCTC3598480.4472970238168561No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCT3541410.44020312829729563No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT3505830.4357804753695585No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT3455820.42956414954279803No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT3302850.4105497251932769No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA3231510.40168204503968585No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT3211610.39920844208122686No Hit
TTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCGTA3150830.3916533874171497No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATC3013860.37462778956689213No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT2789950.34679540572626155No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATC2773310.34472702616702755No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCCTGTAGGCACCATCAATATC2718130.3378680607776926No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAT2559330.3181289577725024No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA2548900.31683249149829495No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT2512210.3122718637321714No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2465840.3065079959339934No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT2457370.30545516090594577No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA2389770.2970523689465575No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATA2311950.2873792140607647No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA2306160.2866595074713437No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2228870.277052232463339No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT2198700.2733020514956653No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTC2182210.2712523171848618No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCT2143310.2664169827585275No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2136050.26551455273448665No Hit
TTTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCGT2107960.2620229192117172No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTC2028760.2521782280403629No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATATCT2002920.24896627324405232No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCT1937640.24085186112705728No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATAT1840950.2288331339886956No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTC1800120.22375789736588753No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATA1699100.2112009440561627No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1634800.2032083475622475No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGATCTGTAGGCACCATCAATAT1582780.19674217540651706No Hit
AAAAACCAACCCGGTGAGCTCCCTCCCGGCCTGTAGGCACCATCAATATC1546370.19221635210413057No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1455000.180858909776774No Hit
AATACATGCCGACGGGCGCTGACCCCCCTCTGTAGGCACCATCAATATCT1347800.16753377223170857No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA1263520.15705762864683814No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1257690.156332949991169No Hit
TGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATATC1252880.1557350590248279No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC1208240.15018623309188275No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1204180.14968156836769464No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCT1194620.1484932445343847No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1155930.14368401345585316Illumina PCR Primer Index 6 (96% over 29bp)
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA1117420.13889715667543834No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCG1112220.13825078806317773No Hit
AAAAACCAACCCGGTGAGCTCCCTCCCGGCTCTGTAGGCACCATCAATAT1101690.13694189162334994No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT1064230.13228555158194927No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATATCTCG1058770.13160686453907558No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATATC1054530.13107982551677078No Hit
TGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC1040360.12931847104836056No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAT1002900.1246621310069599No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATC998720.12414255008402729No Hit
TGAACGCACGTTCGTGTGGAACCTGGCGCTCTGTAGGCACCATCAATATC948680.11792249520758073No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA929810.11557692295501183No Hit
CTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCG895030.11125371135223781No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC894590.11119901862350808No Hit
AAATACATGCCGACGGGCGCTGACCCCCCTCTGTAGGCACCATCAATATC892640.11095663039391032No Hit
CGCGCTCTCCCTCTCCCCCCGTCCGCCTCTGTAGGCACCATCAATATCTC875570.1088348011225086No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA872280.10842584867359753No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATAT849550.10560047202808134No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCAAT835960.10391121252026941No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA824460.10248174347392376No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC809940.10067688342584216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)