FastQCFastQC Report
Thu 6 Jul 2023
SRR5291507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5291507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81970591
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1193811314.563897678863874No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT42328685.1638861552187665No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC28872933.522352303157116No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT22521082.7474585366842117No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATATCT21345482.6040412469394054No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA17313012.112100179929165No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT16814962.0513405838442718No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC16645082.0306160779053064No Hit
TTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCGT16184621.9744422728390478No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTCGTA15080711.8397708026772677No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA12930021.5773974351362186No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT11760761.4347535935174605No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCAA10294471.2558735851983793No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTCG10064061.2277647235701887No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT9640461.1760876531925943No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT8582651.0470401512659582No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCTCGT7837390.9561221780138195No Hit
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATATC7698220.9391441376822574No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAATA6707670.8183020176101939No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATATC6670420.8137577048822302No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC6048970.7379439291830896No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC5949820.7258481276534898No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC5119490.6245520420854352No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATA5091940.6211910805913305No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT4963820.605561084706587No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC4736700.577853586538128No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATATCT4704890.5739729264609059No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTC4575390.5581745775140258No Hit
TTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCGTA4511920.5504315566054659No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAT4091040.4990863125532424No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCTC4071150.4966598325489687No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA4037280.4925278628282672No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATC3842240.46873396337962236No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTC3488840.4256209400759353No Hit
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATAT3348220.40846600703415686No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3333030.4066129034009282No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3161510.38568832570695993No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT2963610.36154552063678547No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA2803190.341975087138264No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2769170.3378248181716782No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCT2587400.31564979200894133No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT2585130.31537286342122384No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATAT2337560.285170568063856No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2284510.27869873476940044Illumina PCR Primer Index 6 (96% over 29bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATAT2278060.2779118671963705No Hit
TTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCG1902440.2320881156023384No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1895080.23119023260427635No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATA1818690.22187103665020544No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCG1754690.21406335840618743No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1710540.2086772803675406No Hit
CGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATATCTCGTAT1677380.20463192707735875No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA1618680.19747082218792344No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTCG1608680.19625087246229567No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1564140.19081721638434934No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGTA1538080.18763802739936328No Hit
AAAACCAACCCGGTGAGCTCCCTCCCGGCCTGTAGGCACCATCAATATCT1434050.17494689040365707No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1380690.16843723866770705No Hit
TAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGTA1366940.16675980779496882No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCTGTAGGCACCATCAATATC1347850.1644309237687453No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAT1314170.1603221330928308No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATATCTC1303740.159049725529001No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCT1294020.15786393439569077No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC1285460.15681965743055334No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1272260.15520932379272462No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1167090.14237911252829688No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATAT1144940.13967692388603128No Hit
AAAAACCAACCCGGTGAGCTCCCTCCCGGCCTGTAGGCACCATCAATATC1142750.1394097548961188No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATCA1111660.1356169311991419No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAT1069070.13042116531769302No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATATCT1059730.12928173227395665No Hit
TGAACGCACGTTCGTGTGGAACCTGGCGCTCTGTAGGCACCATCAATATC1050200.12811912018543334No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1017970.12418722221973488No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1009610.12316734424911002No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA999130.12188883693665206No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT996430.12155945051073257No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATATCTCG989350.12069572610498808No Hit
AAAACCAACCCGGTGAGCTCCCTCCCGGCTCTGTAGGCACCATCAATATC986540.12035292023208664No Hit
ATCCAGCGAAACCACAGCCAAGGGAACCTGTAGGCACCATCAATATCTCG981610.11975148501735214No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA940570.11474481134337558No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATATCTC914490.11156318245893825No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC902110.11005288469861099No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCT899880.109780835909796No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCCTGTAGGCACCATCAATATC875710.10683221742295355No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT861360.10508158956667762No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT856790.10452407254206574No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT853440.1041153893839804No Hit
TGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCGTA849290.10360911024784487No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG834750.10183530334678201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)