FastQCFastQC Report
Fri 7 Jul 2023
SRR5291508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5291508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70303472
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length35-84
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT897093712.760304355949875No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATA54416667.740252145726174No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT41209565.861667827728337No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC26748943.804782216161387No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA17565522.4985280954545175No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT16160732.298710083621475No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT9806601.3948955465528075No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8894131.265105370613844No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT8423071.198101567444635No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8102821.1525490519159565No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC7377471.049374915651392No Hit
TTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCGT7358291.0466467431366688No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6798590.9670347433196471No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT6746970.9596922894505125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT5890010.8377978828698531No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT5709310.8120950271133125No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATA5582140.7940063045534934No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCT5230870.7440414891600233No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT4350070.6187560694015226No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC3305340.4701531668307933No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC3191510.4539619323495147No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA3063760.4357907103080201No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTCGTA2932280.4170889312550595No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATC2869510.4081604959709529No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT2688920.3824732866678334No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2672080.380077956889526No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCTCGT2482980.3531802810535446No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT2451250.3486669904439428No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT2347910.3339678586571087No Hit
TTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCG2284560.32495692389132647No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT2237770.31830149156787024No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA2116380.301034919015095No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAT1998670.2842917914495034No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAAT1910510.27175187023480146No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA1724540.24529940711889736No Hit
TTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCGTA1580200.22476841542050727No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAATA1523850.21675316405425893No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT1487980.21165099783407568No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATATCTC1481930.21079044289590704No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA1459250.20756442868141703No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1435940.204248802960969No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1426930.20296721618528313No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1369710.1948282155965213No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1317970.18746869286910894No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA1315300.18708891077242956No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT1302150.1852184483861622No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1284230.1826694988833553No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATA1227660.17462295461026447No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC1227370.17458170486942665No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT1167500.1660657669936984No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1096770.15600509744383606No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATATCT1064190.15137090242143375No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC1059250.1506682344223341No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA1040110.14794575152703696No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA1023660.14560589553813216No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA978990.13925201304424908No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCCTGTAGGCACCATCAATAT972590.13834167393610375No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATC928610.13208593737731758No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAT892190.12690553889002806No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT846800.12044924324647864No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC820770.11674672340506881No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT818220.11638401016666716No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTC785470.11172563426170475No Hit
TTTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCGT780630.11103719031116985No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATATCT771440.10973000024806741No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT766210.10898608250812991No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC742530.10561782780799218No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAAT741440.10546278567863619No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT732270.10415844042524672No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTCTGTAGGCACCATCAATATCT723120.10285693998157018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)