FastQCFastQC Report
Tue 4 Jul 2023
SRR5291509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5291509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94665378
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length35-84
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT75635087.989729888365312No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA28317352.991310085932367No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA27007822.8529775690538095No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC21254012.245172464213897No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT19103132.017963737492286No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT15830761.6722861445712496No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA14681871.5509228727740358No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT12710161.3426408121457034No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC12463911.3166281340998818No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT11312591.1950081686675356No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA10479011.1069527446454606No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATC10434531.1022540891348895No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA9991271.0554302123000026No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT9574591.0114141201654527No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8217680.8680766055780182No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7510810.7934062229171048No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA7013480.7408706486124209No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT6063820.6405530858388375No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA6022060.6361417581832294No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA5291350.5589530313817582No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC4994670.5276131681426339No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT4917060.5194148171045174No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT4867040.5141309423599407No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC4759600.5027814920889029No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCT4721650.49877263470072447No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA4580110.4838210227185698No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTC4511550.4765786706096499No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC4275530.4516466410771634No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA4266000.450639937232385No Hit
TTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCG4244880.4484089209467901No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAT3911420.4131837935512178No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC3819250.40344739340712293No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATATCT3804640.4019040625391049No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATATC3701190.39097609687883994No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATAT3290000.34753994221625567No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC3251690.343493056141391No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAT3022870.31932160034263No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT3004630.3173948135505253No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC2962320.3129253865124798No Hit
GCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATATCTC2904180.306783753612646No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCTC2807710.2965931219331317No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT2672730.2823344771305936No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA2621240.276895318582048No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATC2251300.23781661760226636No Hit
TTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCGT2119620.22390656909435253No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2108820.2227657084937642No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT2089360.22071004670788935No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG2085450.22029701291638007No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCT2051080.21666632969024854No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA2046880.2162226616789086No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAATA1958750.20691302790762636No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA1957120.20674084246513016No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA1932300.20411897578859295No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA1907840.201535137798742No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAAT1890080.19965905592221903No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG1878690.19845587052956148No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1870190.19755797098280217No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA1865620.19707521793236807No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATATCT1858580.19633154583716975No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA1835360.19387869554590487No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATATCTC1813320.19155049483877834No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATAT1794780.18959201747443508No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATATCTC1767330.18669233011460643No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTCG1588990.16785334127118787No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCG1562620.16506773997141805No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATATCT1544490.16315257305580083No Hit
TTTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCGT1426350.1506728257082542No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT1419240.1499217591462002No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1413100.1492731587677176No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCT1397130.14758616397221802No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTCG1395410.14740447135805024No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCT1345150.14209524415568275No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATATC1343850.14195791834264898No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1325880.14005965306555898No Hit
TGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATATCTCG1282430.13546980185300692No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAT1267240.1338652025453276No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1264760.13360322714815548No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT1254420.13251095875833296No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA1253800.1324454649090399No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC1250510.13209792496682368No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCG1225220.1294264097271127No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC1206780.12747849588684892No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCTC1193600.12608622341316802No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1180370.12468866917744732No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATATCTCGT1152220.12171503714906204No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATAT1144830.1209343927195854No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA1135470.11994564686574219No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA1076980.1137670416316301No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT1071590.1131976676837439No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATA1067620.11277829577778689No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA1039880.10984797419812764No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATCTC1027330.1085222519261477No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC1024390.10821168431820977No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCAA1020450.10779548146947662No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1017060.10743737800318084No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC993110.10490741398613546No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCG955690.10095454327557854No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA951420.10050348079738297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)