FastQCFastQC Report
Mon 3 Jul 2023
SRR5291510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5291510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75944046
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA22607892.976914082244183No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT21831472.8746782861687405No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT15886402.091855890848902No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA15565832.049644550146828No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA13384581.762426510697099No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA10832181.426336963927363No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC9327231.2281713302449018No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA8929761.1758341134471555No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT8804571.1593496085262562No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT7515380.9895943653041609No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG5813230.7654622457170639No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA5689320.7491462859379392No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5173860.6812726306417753No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA5045070.6643140925096354No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA4975690.6551784191218888No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT4560760.6005421412496248No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTC4286220.5643918418568323No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA4217380.5553272734507719No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC4089200.5384490576127587No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA4068480.5357207331302839No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT4012270.5283192312403266No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA3931070.5176271488090061No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT3738410.4922584714541019No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC3711150.4886689866378729No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC3427950.45137837402026226No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA3280150.43191667718098664No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA3269130.4304656088510217No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3230670.4254013540442657No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT3215800.42344333353005714No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3160480.41615902318398995No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA3049140.4014982293674477No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT3031570.3991846839448085No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCCTGTAGGCACCATCAATA2985470.39311442532308594No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC2969800.39105106409526824No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATC2915440.38389316260553197No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTC2905410.38257245340865825No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC2820070.3713352327844108No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATATCT2737300.3604364192026324No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2732820.35984651120642164Illumina PCR Primer Index 6 (96% over 29bp)
CACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAATATCT2716640.3577159952736782No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATAT2683950.35341151036382756No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT2625890.3457664080736494No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC2614970.3443285073328856No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC2606250.3431802935545467No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2568040.33814895772079356No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC2462130.32420316399787286No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATC2429070.3198499590079781No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTCG2381780.31362300607476196No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATC2357440.310418014863206No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT2277860.29993924737694383No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCT2230700.2937294123096892No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2029490.2672349060780881No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1967100.2590196471755008No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCTGTAGGCACCATCAATATCTC1965020.2587457613201172No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCA1955590.25750405765845025No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1949330.2566797665744593No Hit
ACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAATATCTC1842460.24260756399520772No Hit
TACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAATAT1828400.24075620095352834No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAT1803710.23750512317976843No Hit
ACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAATATC1773860.23357459780323003No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT1726650.2273581789413748No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATC1704860.22448896125444778No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCCTGTAGGCACCATCAATATCT1626860.2142182416775635No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA1582210.20833891309925734No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA1550140.20411606724245374No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGT1532390.20177882015925253No Hit
TTTGGTGACTCTAGATAACCTCGGGCCCTGTAGGCACCATCAATATCTCG1476800.1944589573223423No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT1426740.18786726216825478No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1424560.18758020872367004No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCCTGTAGGCACCATCAATAT1413320.18610017169746262No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC1402810.18471625807242348No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1350910.1778822792770351No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1301030.1713142857835096No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATATC1293500.17032276631666424No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1287230.16949715847375316No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA1266970.16682940490160347No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1253740.16508733285029348No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCTGTAGGCACCATCAATATCTCG1164230.15330102375635873No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATATCTC1161050.15288229441976267No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAT1117660.14716887746539078No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT1089770.14349643683719457No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT1078200.14197294676662342No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATATCTC1066270.14040205337492817No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA1041420.13712990745844644No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG1028280.13539968623741747No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGCTGTAGGCACCATCAATATC1027920.13535228291629342No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA1024670.13492433626725656No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA999480.13160742054749097No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC995430.13107413318484507No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATATCTC988320.13013791759264445No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA986480.1298956339513436No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA985550.12977317537177305No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT980730.1291384975722784No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT962500.12673804606091174No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATATCTCG960540.12647996131256953No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCAT923600.12161585386167074No Hit
TGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG920170.12116420555207184No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC916920.12073625890303501No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATATCT862900.11362312721658258No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA846380.11144784148055531No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT820540.10804533643098235No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT819990.10797291469037612No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA801990.10560274863417206No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATC782980.10309958992703655No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT767840.10110601692198491No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)