FastQCFastQC Report
Fri 29 Sept 2023
SRR5315024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5315024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166282537
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGC83479815.0203594139293175No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGC40712132.448370751042847No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA30903501.8584934147354273No Hit
CGAGCGCCGTTCCGAAGGGACGGGCGATGTGGAATTCTCGGGTGCCAAGG27863871.6756943033651213Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTGGAATTCTCGGGTGC22637551.3613907033424684No Hit
TTCGGGGGACGGGCCCGCCGGCCCCCGGCGCCGCTGGAATTCTCGGGTGC21786971.3102380077349915No Hit
CCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCTGGAATTCTCGGGTGCC19739961.187133679587773No Hit
AAGAACGCAGCTAGCTGCGAGAATTAATGTGGAATTCTCGGGTGCCAAGG18677121.1232159634417895Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAGGA18112141.0892388537468611RNA PCR Primer, Index 1 (100% over 22bp)
AGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGCCAA14989690.9014590630163407No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGTGGAATTCTCGGGTGCCAA13526880.8134877085739918No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGTGGAATTCTCGGGTGCC13498540.8117833804760869No Hit
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGTGGAATTCTCGGGTGCC12111120.7283458755503592No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGCTGGAATTCTCGGGTGCCA10734970.6455861327157885No Hit
AGCGGCCGCCGGGTGTTATAGCCGCCGGGCCCGTGGAATTCTCGGGTGCC10458680.6289704372263697No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCTGGAATTCTCGGGTGCCAAG10113500.6082117931602162No Hit
ATGCCGACTCGCGATCCGGCGGCGTTTGGAATTCTCGGGTGCCAAGGAAC9669950.5815373144084276RNA PCR Primer, Index 1 (100% over 24bp)
AGCGGCCGCCGGGTGTTATAGCCGCCGGGCCCGGTGGAATTCTCGGGTGC9422780.5666728551297001No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGCCTGGAATTCTCGGGTGCC9160330.5508894779492088No Hit
CGAGCGCCGTTCCGAAGGGACGGGCGATTGGAATTCTCGGGTGCCAAGGA9041580.5437480184705145RNA PCR Primer, Index 1 (100% over 22bp)
GCGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAGG8685540.5223362691417199Illumina Small RNA Adapter 2 (100% over 21bp)
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGGAATTCTCGGGTGCCAA8621610.5184916080514216No Hit
CTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGCC7260080.43661109163856454No Hit
GCCCCGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGA7238940.4353397615048416RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCGCCCCCGTGGAATTCT6948790.4178905449343727No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGGGCCAA6933970.41699929079143166No Hit
TCGGGGGACGGGCCCGCCGGCCCCCGGCGCCGCTGGAATTCTCGGGTGCC6478530.38960976401268166No Hit
CGCCCCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAA6330410.3807020336717619No Hit
AGCGCCGTTCCGAAGGGACGGGCGATGGTGGAATTCTCGGGTGCCAAGGA6185940.37201380924324RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGTGCCAAGGAA5738460.34510298576933546RNA PCR Primer, Index 1 (100% over 23bp)
AGCTCAGGGAGGCCAGAAACCTCCCGTGGAGCAGTGGAATTCTCGGGTGC5650410.3398077814990278No Hit
AATACTCCAGCTAGGAATAATGGAATAGTGGAATTCTCGGGTGCCAAGGA5302530.31888676319630604RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCGCCCTGGAATTCTCGG5104020.3069486484921745No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGGGCCAAGGAA5011580.3013894357409281RNA PCR Primer, Index 1 (95% over 23bp)
AAGGGCCGGGCGCCCTGGAACGGGTTCGCCCCGAGTGGAATTCTCGGGTG4962250.2984227983002208No Hit
TGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGCCAAGGA4945780.2974323154571547RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCC4847270.2915080613666605No Hit
ACTGCCTGGGAATACCGAGTGTCGTAGGCGTGGAATTCTCGGGTGCCAAG4612820.2774085651579877No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAATTCTCGGGTGCCAA4538530.2729408681081165No Hit
AGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGGGCCAA4525010.2721277941531527No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCCCCGGAATTCTCGGGTGC4316070.2595624337870188No Hit
GCGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGTGCCAAGGA4260980.25624939797496593RNA PCR Primer, Index 1 (100% over 22bp)
AATAATGGAATAGGACTCCGGTTCTATTTTGTGGAATTCTCGGGTGCCAA4175370.251100931903631No Hit
GCCCCGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGGGCCAAGGA4164110.2504237711985354RNA PCR Primer, Index 1 (95% over 22bp)
CGGCCGCCGGGTGTTATAGCCGCCGGGCCCGTGGAATTCTCGGGTGCCAA4074780.24505158951237316No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTGGAATTCTCGGGTGCC3993540.24016592914985416No Hit
CAGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGT3977450.2391982989771199No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGTGGAATTCTCGGGTGCCA3910980.2352008858272351No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGGGCCAAGGA3721060.22377936174981503RNA PCR Primer, Index 1 (95% over 22bp)
ATGCCGACTCGCGATCCGGCGGCGTTATTGGAATTCTCGGGTGCCAAGGA3662600.2202636588350826RNA PCR Primer, Index 1 (100% over 22bp)
AGCCCGGCGCGGGCGAGGCCGCGGGGGGCGTGGAATTCTCGGGTGCCAAG3634080.21854850578807322No Hit
GCCCCGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGCCAA3627030.2181245286148118No Hit
TGAGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGCC3440800.20692491599403492No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCCA3311890.19917244827699498No Hit
AAATTACCCACTCCCGACCCGGGGAGGTAGTGTGGAATTCTCGGGTGCCA3224100.1938928800442827No Hit
CGTCGGATCCCCGCCTCCGCCTCCCCTTGGAATTCTCGGGTGCCAAGGAA3155520.1897685744354502RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC3054020.18366450591260824No Hit
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTG3050790.18347025821478777No Hit
CGAGCTCAGGGAGGCCAGAAACCTCCCGTGGAGCAGTGGAATTCTCGGGT3022420.18176412595869884No Hit
AAAGGGAGTCGGGTTCAGATCCCCGAATCCGGTGGAATTCTCGGGTGCCA3006050.1807796569762464No Hit
GTCGGATCCCCGCCTCCGCCTCCCCTCCGTCCCTTGGAATTCTCGGGTGC3004700.18069846985796229No Hit
CGAGCGCCGTTCCGAAGGGACGGGCGATGTGGAATTCTCGGGGGCCAAGG2917050.17542732103010913Illumina Small RNA Adapter 2 (95% over 21bp)
CGAGCGCCGTTCCGAAGGGACGGGCGATGGTGGAATTCTCGGGTGCCAAG2845750.17113943841258566No Hit
GTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGCCAAGG2710610.16301230717931614Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGGGCCAAGGA2610880.15701468398933557RNA PCR Primer, Index 1 (95% over 22bp)
AAACTAACGAGGATTCCCTCAGTAACGTGGAATTCTCGGGTGCCAAGGAA2579010.15509806661176934RNA PCR Primer, Index 1 (100% over 23bp)
GCGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGGGCCAAGGA2571560.15465003399605337RNA PCR Primer, Index 1 (95% over 22bp)
ATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGGAATTCTCGGGTGCCA2568150.15444496134912833No Hit
AGCTCAGGGAGGCCAGAAACCTCCCGTGGAGCAGAAGTGGAATTCTCGGG2562590.15411059069901006No Hit
CTAATACATGCCGCGTGCATTTATCAGTGGAATTCTCGGGTGCCAAGGAA2545790.153100262115919RNA PCR Primer, Index 1 (100% over 23bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACTGGAATTCTCGGGTG2480990.149203280438282No Hit
AGAAGCCGGCGGGAGCCCCGGGGAGTGGAATTCTCGGGTGCCAAGGAACT2427100.1459624109535928RNA PCR Primer, Index 1 (100% over 25bp)
GATCGGGGATTGCAATTATTCCCCATGTGGAATTCTCGGGTGCCAAGGAA2358290.14182427346534893RNA PCR Primer, Index 1 (100% over 23bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTTGGAATTCTCGGGTG2329220.14007604418496455No Hit
CGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCCAAGGAA2246410.13509596621081144RNA PCR Primer, Index 1 (100% over 23bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA2242720.13487405475416822No Hit
CTAATACATGCCGCGTGCATTTATCAGACTGGAATTCTCGGGTGCCAAGG2196520.13209565115066774Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGGAATACTCCAGCTAGGAATAATGGTGGAATTCTCGGGTGCCAAG2183320.1313018215496676No Hit
AACGCAGCTAGCTGCGAGAATTAATGTGTGGAATTCTCGGGTGCCAAGGA2161760.1300052332013674RNA PCR Primer, Index 1 (100% over 22bp)
CCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAGGAAC2133650.1283147369828739RNA PCR Primer, Index 1 (100% over 24bp)
CCGCTCGTGGTCGGGACCGGAGCGCGGACAGTGGAATTCTCGGGTGCCAA2076950.12490487801494152No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTTGGAATTCTCGGGTGC2060370.12390777992520045No Hit
ATGAAGAACGCAGCTAGCTGCGAGAATTTGGAATTCTCGGGTGCCAAGGA1999900.12027119841213393RNA PCR Primer, Index 1 (100% over 22bp)
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGCGTGGAATTCTCGGGTGCC1984050.1193180015048724No Hit
TTCGGGGGACGGGCCCGCCGGCCCCCGGCGCCGCTTGGAATTCTCGGGTG1935050.11637120980419009No Hit
TGAGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGGGCC1895100.11396867248904194No Hit
CTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCTGGAATTCTCGGGTGCCA1892250.11379727746155328No Hit
ACTGCCTGGGAATACCGAGTGTCGTAGGCGTTGGAATTCTCGGGTGCCAA1891470.11375036934876692No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCCCCCTGGAATTCTCGGGTGC1882810.1132295690196259No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGCTGGAATTCTCGGGGGCCA1869860.11245077407015987No Hit
AGTCGGCCCGGAGGATTCAACCCGGCGGGCCTGGAATTCTCGGGTGCCAA1868630.11237680358461215No Hit
AGCCCGGCGCGGGCGAGGCCGCGGGGGGCGCCGTGGAATTCTCGGGTGCC1865820.11220781410136893No Hit
CCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCTGGAATTCTCGGGGGCC1848430.11116200374065739No Hit
AAATTACCCACTCCCGACCCGGGGAGGTAGTGGAATTCTCGGGTGCCAAG1824580.10972769798430486No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGC1820050.1094552700985071No Hit
TACCCTACGCCGGCAGGCGCGGGTAACCCGTTGTGGAATTCTCGGGTGCC1799550.10822242867271144No Hit
CCGGAATACTCCAGCTAGGAATAATGGAATAGTGGAATTCTCGGGTGCCA1779160.10699620249359078No Hit
AAGCCGGCGGGAGCCCCGGGGAGAGTGGAATTCTCGGGTGCCAAGGAACT1759730.10582770937636102RNA PCR Primer, Index 1 (100% over 25bp)
CCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCTGGAATTCTCGGGTGCCA1751470.10533096448967459No Hit
AAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGAATTCTCGGGTGC1712630.10299518102733782No Hit
CGCGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAG1683120.10122049076025344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)