FastQCFastQC Report
Thu 28 Sept 2023
SRR5315025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5315025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42485
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACCGGGGCGGACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA8512.0030599034953513RNA PCR Primer, Index 1 (100% over 32bp)
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTG7671.8053430622572673No Hit
CCCCTGTCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG5661.3322349064375663RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC4671.0992114864069673RNA PCR Primer, Index 4 (100% over 50bp)
CCCCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAAGG3490.8214663999058491Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCTGTCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA3060.720254207367306RNA PCR Primer, Index 1 (100% over 28bp)
CTTCTGGCGCCAAGCGCCCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC3040.7155466635283041RNA PCR Primer, Index 1 (100% over 27bp)
CTGGCGCCAAGCGCCCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2490.5860892079557491RNA PCR Primer, Index 1 (100% over 30bp)
CGCGCCGGCTGAGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2210.5201835942097212RNA PCR Primer, Index 1 (100% over 33bp)
CCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGC1720.40484877015417203No Hit
CCCCTGTCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1590.3742497352006591RNA PCR Primer, Index 1 (100% over 31bp)
CGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1560.3671884194421561RNA PCR Primer, Index 1 (100% over 30bp)
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1520.35777333176415205RNA PCR Primer, Index 1 (100% over 32bp)
CGGGCCGGCGCCCCGCCTCGGCCGGCGTGGAATTCTCGGGTGCCAAGGAA1440.33894315640814404RNA PCR Primer, Index 1 (100% over 23bp)
TTCGGGGGACGGGCCCGCCGGCCCCCGGCGCCGCTTGGAATTCTCGGGTG1430.33658938448864306No Hit
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGTGGAATTCTCGGGTGCCAAG1390.32717429681063903No Hit
CGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAAGGAAC1390.32717429681063903RNA PCR Primer, Index 1 (100% over 24bp)
CGACCGGGGCGGACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1370.32246675297163707RNA PCR Primer, Index 1 (100% over 33bp)
CCCCGAATCCGGAGCGGCGGAGACGGGCGTGGAATTCTCGGGTGCCAAGG1340.315405437213134Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAG1270.29892903377662705No Hit
CCCCTGTCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1230.289513946098623RNA PCR Primer, Index 1 (100% over 32bp)
TTCGGGGGACGGGCCCGCCGGCCCCCGGCGCCGCTGGAATTCTCGGGTGC1230.289513946098623No Hit
CGGCGCCCCGCCTCGGCCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1190.28009885842061905RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGTGGAATTCTCGGGTGCCAA1160.27303754266211605No Hit
ACCTCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA1150.270683770742615No Hit
CGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA1140.26832999882311404RNA PCR Primer, Index 1 (100% over 28bp)
CGGATCCCCGCCTCCGCCTCCCCTTGGAATTCTCGGGTGCCAAGGAACTC1130.26597622690361306RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAGGA1130.26597622690361306RNA PCR Primer, Index 1 (100% over 22bp)
CCGACCGGGGCGGACTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1120.2636224549841121RNA PCR Primer, Index 1 (100% over 31bp)
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGTGGAATTCTCGGGTGCC1070.25185359538660707No Hit
AGGCCGGAAGGCCCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1060.24949982346710603RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGTCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1000.2353771919501RNA PCR Primer, Index 1 (100% over 30bp)
ACGGCCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1000.2353771919501RNA PCR Primer, Index 1 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTGC950.22360833235259503No Hit
TCCGACCGGGGCGGACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC940.22125456043309405RNA PCR Primer, Index 1 (100% over 31bp)
CCGGCGCCCCGCCTCGGCCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC900.21183947275509002RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGGCGGCCCGTTCGGGGGACTGGAATTCTCGGGTGCCAAGGAACTCC890.20948570083558904RNA PCR Primer, Index 1 (100% over 27bp)
AGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGCCAA860.20242438507708602No Hit
TGGGCGCGCGCCGCGGCTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT840.19771684123808406RNA PCR Primer, Index 1 (100% over 30bp)
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGCTGGAATTCTCGGGTGCCAA820.19300929739908204No Hit
CGCGCCGGCTGAGGTGGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCA820.19300929739908204RNA PCR Primer, Index 1 (100% over 28bp)
CGGGCCGGCGCCCCGCCTCGGCCGGCGCTGGAATTCTCGGGTGCCAAGGA790.18594798164057902RNA PCR Primer, Index 1 (100% over 22bp)
CCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAAGGAA780.18359420972107804RNA PCR Primer, Index 1 (100% over 23bp)
GCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG770.18124043780157703RNA PCR Primer, Index 1 (100% over 29bp)
CCGCGGGGGGCGCCGGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG760.17888666588207602RNA PCR Primer, Index 1 (100% over 29bp)
AGTGCGCCCCGACCGCGCGGCGCCGCCGGTGGAATTCTCGGGTGCCAAGG760.17888666588207602Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCCGGCTGAGGTGGGATCCCGGGGTGGAATTCTCGGGTGCCAAGGAA730.17182535012357303RNA PCR Primer, Index 1 (100% over 23bp)
CGCGCCGGCTGAGGTGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC710.16711780628457104RNA PCR Primer, Index 1 (100% over 31bp)
AGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC710.16711780628457104RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGGCTGAGGTGGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT680.16005649052606802RNA PCR Primer, Index 1 (100% over 30bp)
TGGGCGCGCGCCGCGGCTGGACGTGGAATTCTCGGGTGCCAAGGAACTCC680.16005649052606802RNA PCR Primer, Index 1 (100% over 27bp)
CCGGCGCGCGGCTCGGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA680.16005649052606802RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA670.157702718606567RNA PCR Primer, Index 1 (100% over 32bp)
TGGGGCGCGGGAGGTGTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG670.157702718606567RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGGCTGAGGTGGGATCCCGTGGAATTCTCGGGTGCCAAGGAACTCCA670.157702718606567RNA PCR Primer, Index 1 (100% over 28bp)
ATAGCCGCCGGGCCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC670.157702718606567RNA PCR Primer, Index 1 (100% over 33bp)
CCCTGTCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG650.15299517476756502RNA PCR Primer, Index 1 (100% over 29bp)
CCCCTGTCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT650.15299517476756502RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAGG650.15299517476756502Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGCCGAGGCGCCTCGGTGGGCTCGTGGAATTCTCGGGTGCCAAGGAAC630.14828763092856304RNA PCR Primer, Index 1 (100% over 24bp)
AGCCCGGCGCGGGCGAGGCCGCGGTGGAATTCTCGGGTGCCAAGGAACTC620.14593385900906203RNA PCR Primer, Index 1 (100% over 26bp)
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA610.14358008708956102No Hit
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCTGGAATTCTCGGGTGCCA590.13887254325055903No Hit
TGGGCGCGCGCCGCGGCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG580.13651877133105803RNA PCR Primer, Index 1 (100% over 29bp)
CGGTGGGGGGCGGGGCGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG580.13651877133105803RNA PCR Primer, Index 1 (100% over 29bp)
CGACCGCGCGGCGCCGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC570.13416499941155702RNA PCR Primer, Index 1 (100% over 31bp)
AGCGGCCGCCGGGTGTTATAGCCGCCGGGCCCGGTGGAATTCTCGGGTGC570.13416499941155702No Hit
CGGATCCCCGCCTCCGCCTCCCTGGAATTCTCGGGTGCCAAGGAACTCCA560.13181122749205604RNA PCR Primer, Index 1 (100% over 28bp)
CGTCGGATCCCCGCCTCCGCCTCCCCTTGGAATTCTCGGGTGCCAAGGAA560.13181122749205604RNA PCR Primer, Index 1 (100% over 23bp)
CGGATCCCCGCCTCCGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG560.13181122749205604RNA PCR Primer, Index 1 (100% over 29bp)
CGGATCCCCGCCTCCGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC550.129457455572555RNA PCR Primer, Index 1 (100% over 31bp)
CTGTCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA540.12710368365305402RNA PCR Primer, Index 1 (100% over 32bp)
CGCGCCGGCTGAGGTGGGATCCCGTGGAATTCTCGGGTGCCAAGGAACTC540.12710368365305402RNA PCR Primer, Index 1 (100% over 26bp)
CGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA530.12474991173355301RNA PCR Primer, Index 1 (100% over 28bp)
CTGGCGCCAAGCGCCCGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAG530.12474991173355301RNA PCR Primer, Index 1 (100% over 29bp)
CTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGCC530.12474991173355301No Hit
TCAGTGCGCCCCGACCGCGCGGCGCCGCCGTGGAATTCTCGGGTGCCAAG520.12239613981405201No Hit
CGGCGGCGGGCGCGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC520.12239613981405201RNA PCR Primer, Index 1 (100% over 31bp)
GTGGGGCGCGGGAGGTGTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA520.12239613981405201RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGGCTGAGGTGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC520.12239613981405201RNA PCR Primer, Index 1 (100% over 33bp)
CCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGTGGAATTCTCGGGTG500.11768859597505No Hit
AGCCGGCCGCCGCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT500.11768859597505RNA PCR Primer, Index 3 (100% over 34bp)
ACCGAGTGTCGTAGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA500.11768859597505RNA PCR Primer, Index 1 (100% over 32bp)
CCCCGAATCCGGAGCGGCGGAGACGGGCTGGAATTCTCGGGTGCCAAGGA490.11533482405554903RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGCGCGGGAGGTGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCA480.11298105213604802RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGTCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA480.11298105213604802RNA PCR Primer, Index 1 (100% over 32bp)
CGCCGGCTGAGGTGGGATCCCGGGGTGGAATTCTCGGGTGCCAAGGAACT480.11298105213604802RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGACCGCGCGGCGCCGCCGTGGAATTCTCGGGTGCCAAGGAACTCCA460.10827350829704602RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGTGCCAAGGAA460.10827350829704602RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG450.10591973637754501RNA PCR Primer, Index 1 (100% over 29bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC450.10591973637754501RNA PCR Primer, Index 1 (100% over 27bp)
ACTGCCTGGGAATACCGAGTGTCGTAGGCGTTGGAATTCTCGGGTGCCAA450.10591973637754501No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCGGCTGGAATTCTCGGGTGCCAAGG440.10356596445804402Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGCCGCCGGGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA440.10356596445804402RNA PCR Primer, Index 1 (100% over 32bp)
CGGCGGCGGCGGGGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC440.10356596445804402RNA PCR Primer, Index 1 (100% over 31bp)
ACCGCATGTTCGTGGGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT430.10121219253854301RNA PCR Primer, Index 1 (100% over 30bp)
CCGCCGGAATACTCCAGCTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG430.10121219253854301RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph