FastQCFastQC Report
Tue 27 Jun 2023
SRR533660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR533660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11271618
Total Bases394.5 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA698730.6199021294014754Illumina Single End Adapter 1 (100% over 21bp)
AAAGTCTTTGGGTTCCGGGGGGAGTTCGTATGCCG206490.18319463984673717No Hit
GTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA201100.1784127176772669No Hit
AGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG190270.168804514134528No Hit
TACCTGGTTGATTCTGCCAGTAGTCGTATGCCGTC164200.145675625274029No Hit
ATCTGCGGCCACAGAACCGTGAAAGCATCGTATGC151040.13400028283428342No Hit
TACCTGGTTGATCCTGCCAGTAGTCGTATGCCGTC124800.11072057268086977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)