FastQCFastQC Report
Tue 27 Jun 2023
SRR533662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR533662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12099322
Total Bases423.4 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCTTTGGGTTCCGGGGGGAGTTCGTATGCCG2734102.259713395510922No Hit
AGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG1966321.6251489133027455No Hit
CTTTGGGTTCCGGGGGGAGTTCGTATGCCGTCTTC1936761.6007177922862124No Hit
TCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA1250981.0339257026137498Illumina Single End Adapter 1 (100% over 21bp)
GAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCC938630.7757707415341124No Hit
TACCTGGTTGATCCTGCCAGTAGTCGTATGCCGTC692600.5724287691492135No Hit
GGGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAA536050.4430413538874327Illumina PCR Primer Index 2 (96% over 26bp)
ATCTGCGGCCACAGAACCGTGAAAGCATCGTATGC403800.3337377086087964No Hit
GTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA319710.2642379465560136No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCC308710.25514652804512516No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACCCAC255280.21098702885996423No Hit
AGGCGCGGGTAACCCGTTGAACTCGTATGCCGTCT232350.19203555372772127No Hit
AGCGCATGTTTGACTGTAGTCTGTAAATTCGTATG230160.19022553495146255No Hit
GAGCTGAGTTTGTGTCTGGCTGGAAACTCGTATGC199280.16470344371362297No Hit
GTGGACTCGTATGCCGTCTTCTGCTTGAAAAAAAA190120.15713277157182856TruSeq Adapter, Index 21 (96% over 25bp)
TACCTGGTTGATTCTGCCAGTAGTCGTATGCCGTC183410.1515870062801866No Hit
AAGACTTAAAACTCGTATGCCGTCTTCTGCTTGAA179200.14810747246829203RNA PCR Primer, Index 13 (96% over 26bp)
AAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGAT172730.1427600653987058No Hit
TCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAA166420.13754489714382342Illumina Single End Adapter 1 (100% over 21bp)
AGAGGTCTTGGGGCCGAAACTCGTATGCCGTCTTC165400.1367018747000865No Hit
GTTGAACTCGTATGCCGTCTTCTGCTTGAAAAAAA165220.13655310603354467Illumina PCR Primer Index 5 (96% over 26bp)
CGGAAGATGTAACGGGGCTAAAATCGTATGCCGTC149760.12377553056278691No Hit
TCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAA145730.12044476541743414Illumina Single End Adapter 1 (100% over 21bp)
CGGATGTGCTGACCCCTGCGATTTCCCCTCGTATG137680.11379150005264757No Hit
TGCGCGGAAGATGTAACGGGGCTAAAATCGTATGC135000.11157649990635839No Hit
TCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAC134330.11102274986978608Illumina Single End Adapter 1 (100% over 21bp)
CGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA130620.10795646235384097Illumina Paired End PCR Primer 2 (100% over 26bp)
AGAGGTCTTGGGGCCGAAACGATCTCAACTCGTAT128040.1058241114667417No Hit
AAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCTC127550.10541913009671121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)