FastQCFastQC Report
Tue 27 Jun 2023
SRR533663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR533663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11058919
Total Bases387 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGGTTCCGGGGGGAGTTCGTATGCCGTCTTC3187582.8823612868491035No Hit
AGGCGCGGGTAACCCGTTGAACTCGTATGCCGTCT2570622.3244767413523872No Hit
TCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA690820.6246722667920798Illumina Single End Adapter 1 (100% over 21bp)
GTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA639000.577814160678815No Hit
AAAGTCTTTGGGTTCCGGGGGGAGTTCGTATGCCG627020.5669812754754782No Hit
CGGAAGATGTAACGGGGCTAAAACCGTATGCCGTC518690.4690241424139195No Hit
CGGAAGATGTAACGGGGCTAAAATCGTATGCCGTC507120.4585619986908304No Hit
GCGGAAGATGTAACGGGGCTAAAACCGTATGCCGT371860.33625348010958395No Hit
TGCGCGGAAGATGTAACGGGGCTAAAACCGTATGC347490.3142169682226626No Hit
TACCTGGTTGATCCTGCCAGTAGTCGTATGCCGTC330720.29905273743301675No Hit
GAAGATGTAACGGGGCTAAAACCGTATGCCGTCTT320690.2899831348796388No Hit
GCGGAAGATGTAACGGGGCTAAAATCGTATGCCGT320210.28954909607349505No Hit
GAAGATGTAACGGGGCTAAAATCGTATGCCGTCTT310160.2804614085698611No Hit
TGCGCGGAAGATGTAACGGGGCTAAAATCGTATGC277740.2511457042049047No Hit
CTGCGCGGAAGATGTAACGGGGCTAAAACCGTATG273680.2474744593029391No Hit
GATGTAACGGGGCTAAAACCGTATGCCGTCTTCTG270920.24497873616761276No Hit
TAACGGGGCTAAAATCGTATGCCGTCTTCTGCTTG253510.22923578696977526Illumina Single End Adapter 1 (95% over 22bp)
TAACGGGGCTAAAACCGTATGCCGTCTTCTGCTTG249370.22549220226678576Illumina Paired End PCR Primer 2 (95% over 21bp)
TTTGGGTTCCGGGGGGAGTTCGTATGCCGTCTTCT246150.2225805252755717No Hit
GGAAGATGTAACGGGGCTAAAACCGTATGCCGTCT237930.21514761072036065No Hit
GGAAGATGTAACGGGGCTAAAATCGTATGCCGTCT236100.21349283777193773No Hit
CTGCGCGGAAGATGTAACGGGGCTAAAATCGTATG233960.21155774809454705No Hit
AGATGTAACGGGGCTAAAACCGTATGCCGTCTTCT226110.20445940511907176No Hit
GATGTAACGGGGCTAAAATCGTATGCCGTCTTCTG225590.20398919641241609No Hit
AAGATGTAACGGGGCTAAAACCGTATGCCGTCTTC214700.19414194099803064No Hit
ATGTAACGGGGCTAAAACCGTATGCCGTCTTCTGC213290.1928669520049835No Hit
GGGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAA212560.19220685132063994Illumina PCR Primer Index 2 (96% over 26bp)
GTTGAACTCGTATGCCGTCTTCTGCTTGAAAAAAA207380.18752284920433904Illumina PCR Primer Index 5 (96% over 26bp)
AGATGTAACGGGGCTAAAATCGTATGCCGTCTTCT202550.18315533371751797No Hit
AAGATGTAACGGGGCTAAAATCGTATGCCGTCTTC198930.17988195772118415No Hit
TGTAACGGGGCTAAAACCGTATGCCGTCTTCTGCT192610.17416711344029195No Hit
CCTGCGCGGAAGATGTAACGGGGCTAAAACCGTAT192470.17404051878850002No Hit
AACGGGGCTAAAACCGTATGCCGTCTTCTGCTTGA185830.16803631530351204Illumina Paired End PCR Primer 2 (95% over 21bp)
ATGTAACGGGGCTAAAATCGTATGCCGTCTTCTGC178140.16108265193008467No Hit
AACGGGGCTAAAATCGTATGCCGTCTTCTGCTTGA166850.15087369751057947Illumina Single End Adapter 1 (95% over 22bp)
TGTAACGGGGCTAAAATCGTATGCCGTCTTCTGCT165910.15002370484854805No Hit
CCTGCGCGGAAGATGTAACGGGGCTAAAATCGTAT164800.14901999010934072No Hit
ACGGGGCTAAAACCGTATGCCGTCTTCTGCTTGAA153130.13846742163497175Illumina DpnII expression Adapter 2 (95% over 21bp)
GTGGACTCGTATGCCGTCTTCTGCTTGAAAAAAAA149330.13503128108633403TruSeq Adapter, Index 21 (96% over 25bp)
ACGGGGCTAAAATCGTATGCCGTCTTCTGCTTGAA148520.13429884060096653Illumina Single End Adapter 1 (95% over 22bp)
AGGCGCGGTAACCCGTTGAACTCGTATGCCGTCTT143300.12957866858415365No Hit
GCGCGGAAGATGTAACGGGGCTAAAACCGTATGCC139620.12625103773705187No Hit
TACCTGGTTGATTCTGCCAGTAGTCGTATGCCGTC136260.1232127660940459No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAG127610.11539102510833112No Hit
CGGGGCTAAAACCGTATGCCGTCTTCTGCTTGAAA122670.11092404239510208Illumina Single End Adapter 1 (95% over 21bp)
ATTGATTCGTATGCCGTCTTCTGCTTGAAAAAAAA120800.10923309954616722Illumina Single End Adapter 1 (95% over 22bp)
AGGTTTGTGTGGAACTCGTATGCCGTCTTCTGCTT113670.1027858147799075RNA PCR Primer, Index 30 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)