FastQCFastQC Report
Tue 27 Jun 2023
SRR533666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR533666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85431283
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNAAA3338860.3908240497804534No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNAAA3023370.353894954381055No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTANAAA1987900.23268993864928844No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCANAAA1454750.17028305661756243No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCANAAA958990.11225279152134471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)