FastQCFastQC Report
Tue 27 Jun 2023
SRR533667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR533667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84879959
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNAAA2359040.2779266186968823No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNAAA1436430.1692307603494483No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAA1366660.16101091660517883No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTANAAA1264000.14891618880258883No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)