FastQCFastQC Report
Tue 4 Jul 2023
SRR5345621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5345621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69352081
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG25084693.6170060996439317No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG14304272.06255815164364Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAA8888031.2815808656123815No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA6820620.9834773379042513No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAG4058030.5851345686368085No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA3590780.5177609594728672No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG3526900.5085499885720806No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG3435370.4953521149567235No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGC3369300.48582536405792925No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCCA3351640.4832789372246811No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGG3337740.4812746714839025Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCCGCCCCACCCCGCGCGCGCCGCTTGGAATTCTCGGGTGCCAAGGA3323090.4791622619081899RNA PCR Primer, Index 1 (100% over 22bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAA3242130.4674884954065041No Hit
TCCCCCGCCCCACCCCGCGCGCGCCGCTGGAATTCTCGGGTGCCAAGGAA3234340.4663652414409887RNA PCR Primer, Index 1 (100% over 23bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGG2778860.4006887695266131Illumina Small RNA Adapter 2 (100% over 21bp)
CGCACCGCACGTTCGTGGGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG2583050.37245457710201946No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGTGGAATTCTCGGGTGCCA2489680.35899139061162416No Hit
CGCGCTGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG2388300.3443732279641328Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGA2341240.33758756280146807RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2306690.3326057368055041No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGTGGAATTCTCGGGTGCCAAGGA2130600.30721500628077764RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2104760.3034890906878483RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT2070190.29850438085628606No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA2039650.2941007638977697No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGTGGAATTCTCGGGTGCCAA2011480.29003888145764506No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAG1781190.25683295646168136No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG1757460.2534112855243666No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAA1623440.23408670318054337No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA1618760.23341188565055457No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1596940.23026562101287196No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTGGGGTGCCAAG1566590.22588940049253894No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGA1547470.22313245366061907RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGGGT1546900.22305026434606914No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1504280.2169048106862143No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG1422610.20512866802079088No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCTTGGAATTCTCGGGTGCCAA1370100.1975571576576051No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGTGGAATTCTCGGGTGCCAA1361210.19627529273418629No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGTGTGCCAAG1263560.1821949654257671No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC1217820.17559963341258641RNA PCR Primer, Index 1 (100% over 24bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC1167000.16827180715745213No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCTGGTGCCAAG1166300.16817087291151364No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC1157000.16682988935833087No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG1125450.16228063870210324No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAA1118080.1612179452841509No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG1107740.15972700227985948No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC1080990.15586987216721007No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTT1043450.15045691274930884No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC1018890.146915562634667No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGG961480.1386375125499118Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGGAATTCTCGGGTG934970.13481498846444132No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA915690.1320349709477355No Hit
CCCCCGCCCCACCCCGCGCGCGCCGCTTGGAATTCTCGGGTGCCAAGGAA912370.13155625423842723RNA PCR Primer, Index 1 (100% over 23bp)
GCGCGCTGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG901610.13000475068657277No Hit
ACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAG890610.12841864110753937No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA880560.1269695137194225No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA875250.1262038553680891RNA PCR Primer, Index 1 (100% over 22bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC846900.1221160184075803No Hit
CCCCCGCCCCACCCCGCGCGCGCCGCTGGAATTCTCGGGTGCCAAGGAAC822310.11857034253954109RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC794480.1145574853045866No Hit
CACCCCCGCGGGGAATCCCCCGCGAGGGGGGTTGGAATTCTCGGGTGCCA789920.11389997078818731No Hit
AGGGGTGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAA771330.11121944559962087No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG750620.10823323383764073No Hit
GGTGGCGGCGGCGGCGGGGGCGGCGGGATGGAATTCTCGGGTGCCAAGGA749080.10801117849657604RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG744560.10735943165137322No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTGGGGTGCCAAGG711120.1025376585311117Illumina Small RNA Adapter 2 (95% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAT700110.10095010703427917No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC696070.1003675722434342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)