FastQCFastQC Report
Thu 6 Jul 2023
SRR5345622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5345622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47613077
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG25843775.42787226290794No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG14471893.0394779988699323Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG7300121.5332174394022047No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA5956041.250925244759964No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA5816861.2216937796311715No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAA5378671.1296623404532329No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC5317181.1167478211920645No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCCA5274551.1077943985850778No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG4650730.9767757710765049No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC4583660.962689304873113No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAA4211360.8844965008247629No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTT3138230.6591109413071539No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGG3113400.6538959874405933Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC3095350.6501050121167342No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGC3000720.6302302201556937No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTT2805850.5893023885013775No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGTGGAATTCTCGGGTGCCA2537120.5328620118376303No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAA2528310.5310116798374531No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT2424560.5092214477127786No Hit
CGCGCTGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG2286960.48032182419128255Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAG2169730.4557004370879034No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGG2167420.45521527625698294Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTGGGGTGCCAAG2009130.42197020789057593No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGTGGAATTCTCGGGTGCCAA1949070.4093560262866439No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAA1833820.3851504913240537No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGA1818880.38201269789810055RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGGGT1688330.3545937600294138No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC1648490.3462263108935388RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAG1613500.338877489476263No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA1558750.3273785477044468No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC1545380.32457049562245266No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAG1500120.31506470375775125No Hit
TCCCCCGCCCCACCCCGCGCGCGCCGCTGGAATTCTCGGGTGCCAAGGAA1492910.313550413891545RNA PCR Primer, Index 1 (100% over 23bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG1440890.30262484401081663No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC1331700.2796920686306411No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1329620.27925521385647895No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGTGTGCCAAG1327880.27888976803578563No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG1320520.27734397421951956No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA1299940.2730216322713191RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGC1298240.272664587503975No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC1235110.2594056250554863No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCTGGTGCCAAG1178800.24757904220304855No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCTTGGAATTCTCGGGTGCCAA1164440.24456306405065986No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1140750.2395875402045535No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGA1130580.23745157239050105RNA PCR Primer, Index 1 (100% over 22bp)
AACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAA1102240.23149942609254176No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG1100120.23105417026503036No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGGATTCTCGGGTG1093250.22961128935229286No Hit
CGCGCTGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCCA1055370.22165549183053218No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1054530.22147906971019746No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGTGGAATTCTCGGGTGCCAA1024990.21527489181176004No Hit
TCCCCCGCCCCACCCCGCGCGCGCCGCTTGGAATTCTCGGGTGCCAAGGA969920.20370874161314967RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCTCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAA938490.19710761394395915No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTGGGGTGCCAAGG883030.18545955347519338Illumina Small RNA Adapter 2 (95% over 21bp)
ACCCCTCCTCCCCGCGCCCCCGCCCCGGTGGAATTCTCGGGTGCCAAGGA872660.1832815803943946RNA PCR Primer, Index 1 (100% over 22bp)
GCGCGCTGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG848760.17826195101820452No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTTCCAAGG823440.17294408424811528Illumina Small RNA Adapter 2 (95% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAGTTCTCGGGTG790180.16595860838819554No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA787810.16546084597725116No Hit
GCGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCC775390.16285231891230217No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGGATTCTCGGGTG757680.15913275254191198No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGTTGCCAAG757650.15912645175190002No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA737230.15483771401709662No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC733020.15395350315208572No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC733010.1539514028887484No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCA726040.15248751934263774No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA661660.13896602397698432No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA657770.1381490215387676RNA PCR Primer, Index 1 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG639380.13428663726143975No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGGATTCTCGGGTT637380.13386658459397616No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGTGTGCCAAGG636480.13367756089361751Illumina Small RNA Adapter 2 (95% over 21bp)
GGTGGCGGCGGCGGCGGCGGCGGCGGGATGGAATTCTCGGGTGCCAAGGA629820.13227878551096373RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA621820.13059857484110932No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGGATTCTCGGGTT620980.1304221527207746No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCTGGTGCCAAGG600490.12611871314260997Illumina Small RNA Adapter 2 (95% over 21bp)
GGTGGCGGCGGCGGCGGGGGCGGCGGGATGGAATTCTCGGGTGCCAAGGA583960.12264697784602327RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGGTGTTGACGCGATGTGATTTCTGCCCATGGAATTCTCGGGTGCCA574340.12062652451552333No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAT565880.11884970173215229No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAGTTCTCGGGTG558420.11728290528251303No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGTGGAATTCTCGGG557100.11700567052198706No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGGTGGAATTCTCGGGTGCCAAGG556880.11695946472856605Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA534560.11227167695967225No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG531980.1117298090186442No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG526800.11064187260991344No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGG524480.11015461151565567Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGTGTGCCA520500.1093187067074031No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCTGGTGCCA520300.10927670144065674No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAGTTCTCGGGTGC514860.10813415818515573No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAG509810.10707352519981014No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCTGGTGCCAA491170.10315863433904933No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGTTGCCAAGG479190.10064251886094235Illumina Small RNA Adapter 2 (95% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG477590.10030647672697147No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGGATTCTCGGGT476470.10007124723319183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)