FastQCFastQC Report
Mon 3 Jul 2023
SRR5345623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5345623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46316070
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG15288083.300815462106349Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG14705753.1750858827184607No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA8794851.8988765670317018No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG8080291.7445975014719513No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAA6026161.3010948467778032No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG4427320.9558928466944626No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA3800290.8205121893977618No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA3592120.7755666661700787No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGC3139300.6777993037837623No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAG3036540.655612619982654No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC2950280.6369884146042616RNA PCR Primer, Index 1 (100% over 24bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAA2707480.5845660048445388No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC2469560.5331972250668073No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG2458420.530792012362016No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGG2427920.5242068249745715Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGA2427620.524142052639613RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCCA2204190.47590177664037553No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGG2138860.46179652116425246Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG2136030.4611855021378109No Hit
CGCGCTGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG1994430.4306129600374125Illumina Small RNA Adapter 2 (100% over 21bp)
CGCACCGCACGTTCGTGGGGAACCTGGCGCTTGGAATTCTCGGGTGCCAA1967420.42478129081331817No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA1849190.3992545136061846RNA PCR Primer, Index 1 (100% over 23bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAG1671680.3609287230112572No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGTGGAATTCTCGGGTGCCA1587380.3427276968879268No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGA1565930.3380964749383961RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCCGCCCCACCCCGCGCGCGCCGCTTGGAATTCTCGGGTGCCAAGGA1403180.30295748322342547RNA PCR Primer, Index 1 (100% over 22bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG1332330.2876604167840665No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG1305300.28182442940430824No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC1217440.262854771572804No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGTGGAATTCTCGGGTGCCAA1183880.25560890636878303No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG1168090.2521997224721355No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGGAATTCTCGGGTG1143060.24679555065876702No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGGGT1131740.2443514745530007No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGC1089240.23517539376721727No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGTGGAATTCTCGGGTGCCAAGG1031280.22266137865324065Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA986220.21293257394247828No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA976040.21073463270955417No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA923500.19939083778049388RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGTGGAATTCTCGGGTGCCAA920950.19884027293334688No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAGG909890.19645233285121128Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC907750.19599029019517414No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC893880.19299564924226084No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC883160.19068111780641148No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA873150.18851988089663047No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG867240.18724386589794859Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGG867160.18722659327529298Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCTGGTGCCAA860390.1857648975830635No Hit
GGTGGCGGCGGCGGCGGGGGCGGCGGGATGGAATTCTCGGGTGCCAAGGA843260.1820663972569348RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCCGCCCCACCCCGCGCGCGCCGCTGGAATTCTCGGGTGCCAAGGAA825720.17827937473969618RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG791850.1709665781228848No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGTGGAATTCTCGGGTGCCAA789930.17055203517915057No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTGGGGTGCCAAG775110.16735228183220208No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGTGGAATTCTCGGGTGCCA773980.16710830603719184No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG771460.1665642184235407Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCTGGAATTCTCGGGTGCCAAG769360.16611081207883138No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCTGGTGCCAAGG735180.1587310840492296Illumina Small RNA Adapter 2 (95% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCTGGTGCCAAG720970.15566303445003No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGTGGAATTCTCGGGTGCCAAGGAA706640.15256907591684699RNA PCR Primer, Index 1 (100% over 23bp)
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTGGAATTCTCGGGTGCCAA706360.15250862173755242No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCTGGTGCCAAG705980.15242657677993837No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA699440.1510145398778437No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAGG678770.14655172599920502Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGTGTGCCAAG660110.14252288676478814No Hit
GGTGGCGGCGGCGGCGGGGGCGGCGGGTGGAATTCTCGGGTGCCAAGGAA655650.14155993805173883RNA PCR Primer, Index 1 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG653770.14115403141933244Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA639540.13808166366446895No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGTGTGCCAA628320.13565917833702212No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGTGTGCCAAG623940.13471350224662845No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTGGGGTGCCAAGG619230.13369657658778045Illumina Small RNA Adapter 2 (95% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA608400.1313582952957796No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGTGTGCCAAGG604120.13043420998370545Illumina Small RNA Adapter 2 (95% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC600020.12954898807260634No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGG588640.1270919574998483Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTT582460.12575764739970383No Hit
GGAGGTGGAGCACGAGCGCACGTGTTAGGTGGAATTCTCGGGTGCCAAGG580960.12543378572491146Illumina Small RNA Adapter 2 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGCCAAGG576220.12441038283256761Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCCTCCTCCCCGCGCCCCCGCCCCGGTGGAATTCTCGGGTGCCAAGGA569280.12291198281719497RNA PCR Primer, Index 1 (100% over 22bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAGTTCTCGGGTG564270.12183028482338851No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAA563700.12170721738696741No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA551180.11900405194136723No Hit
GGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGG550870.1189371205285768Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA550090.11876871245768478No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGATGGAATTCTCGGGTGCCAAGGA548070.11833257873563106RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC544890.11764599198507127No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA537830.11612168303571524No Hit
CCCCCGCCCCACCCCGCGCGCGCCGCTTGGAATTCTCGGGTGCCAAGGAA518890.11203238962200378RNA PCR Primer, Index 1 (100% over 23bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGTGGAATTCTCGGGTGCCAAGG508350.10975672158712949Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGG470050.10148745349076466Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)