FastQCFastQC Report
Mon 3 Jul 2023
SRR5345624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5345624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49755922
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA11828772.377359221682195No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG10879922.186658303709054No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG9026541.8141639501726048Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA7035961.4140949895371249No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC5479241.1012236895137828No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCCA4490600.9025257335197205No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC4487370.9018765645625056No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA4333950.8710420440003102No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGG3226930.6485519452337754Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA3156860.6344691994653421No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA3024090.6077849386450923RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2914220.5857031450447245No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA2757390.5541832789270793No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA2756740.5540526412112311No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2635650.5297158396542225No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG2536040.5096961121532428No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2446220.49164398963403794No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2432300.4888463327038739No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2388330.4800091936794981No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2277130.4576600952143948RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGA2275060.4572440643346937RNA PCR Primer, Index 1 (100% over 22bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAA2253550.4529209608456256No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCTTGGAATTCTCGGGTGCCAA2159060.4339302565833269No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1932940.3884844099562661No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG1886570.37916491628875854No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAA1862140.3742549479838802No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC1794810.36072289043302225No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGTGGAATTCTCGGGTGCCAA1778590.35746297696985696No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGTGGAATTCTCGGGTGCCAAGGA1764120.35455478043397526RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1741200.3499482935920673RNA PCR Primer, Index 4 (100% over 50bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1707020.3430787595494663No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG1704300.342532090953917Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG1598240.3212160353495208No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAA1498940.30125861198994563No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC1443280.2900720038913157RNA PCR Primer, Index 1 (100% over 24bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG1438890.28918969685658724No Hit
CGCGCTGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG1418650.28512183936617636Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGG1387570.2788753467376205Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1378740.27710068361309836RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA1268780.2550008017136131No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1187170.23859873403612136No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA1166470.23443842523911024No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAG1163870.23391587437571754No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA1145750.230274096820073No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1143160.22975355576769335No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA1095320.2201386198812676No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1084820.21802831831756628No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGTGGAATTCTCGGGTGCCAAGGAA1079160.21689076528418066RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGTCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1042780.20957907281870888No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC1025940.20619455107273463No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC1001720.2013267887991303No Hit
ACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAG956650.19226857056331906No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGTGTGCCAA935430.1880037515936294No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG918110.18452275891902878No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCATGGAATTCTCGGGTGCCA907700.18243054565444491No Hit
CGCGCTGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCCA900670.18101764851227156No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGA877750.17641116167036358RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCTGGTGCCAA877580.17637699488314174No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC849950.17082388705408777No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA834200.16765843470853578No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGTGGAATTCTCGGGTGCCA826010.1660123994888488No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG782680.1573038883693081No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG773030.15536442074171591No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG766490.15405000433918198No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG755500.15184122203584127No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG740250.1487762602409418No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC722000.1451083551421276No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCTGGAATTCTCGGGTGCCAA709510.14259810118682958No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA696470.13997730762581387No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGG671790.1350170940456093Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG667100.13407449268048938Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCC656100.13186370056613564No Hit
TCCCTTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA641490.12892736667607124No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA617050.12401538856017984No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGGTGGAATTCTCGGGTGCCAAGGAA594090.11940086247421965RNA PCR Primer, Index 1 (100% over 23bp)
AGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGCCAAGG592640.11910943987732757Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTTTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG589770.11853262411658254No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG586680.11791159251355046Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTGGAATTCTCGGGTGCCAA580210.11661124478810783No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC580050.11657908781189906No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGTGTGCCAAG564520.11345785130863417No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA564150.11338348830115137No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG561920.11293530044524148No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG561500.11285088838269343No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGTGGAATTCTCGGGTGCCAAG556870.11192034588365181No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGTGGAATTCTCGGGTGCCAAGGAA545060.1095467590772411RNA PCR Primer, Index 1 (100% over 23bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA539330.1083951373667641No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTGGGGTGCCAAG532580.10703851493295612No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGACTGGAATTCTCGGGTGCCAAG530030.10652601312462866No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGTGTGCCAA528850.1062888554250889No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCTGGTGCCAAG521780.10486791903886335No Hit
CGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT521010.1047131635908586RNA PCR Primer, Index 1 (100% over 25bp)
AACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAA505030.10150148559200652No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA504430.10138089693122358No Hit
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTTGGAATTCTCGGGTGCCA501520.10079604192642637No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGTTGCCAA499370.10036393255862086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)