FastQCFastQC Report
Fri 7 Jul 2023
SRR5350740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5350740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121707924
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGC2398374919.70598808340532TruSeq Adapter, Index 21 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTGCTTG1390683911.426403920914796Illumina Single End Adapter 2 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGCT113004759.284913117078556TruSeq Adapter, Index 14 (95% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTCGTATGCCGTCTT47111233.8708432821514567No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTCGTATGCCGTCTTC45830093.765579799060577No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTGCT43348053.5616456657333173TruSeq Adapter, Index 15 (95% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTGCTTGA35448892.9126197239220017Illumina Single End Adapter 2 (95% over 22bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCGTATGCCGTCTTCT24028861.974305304887133No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGCCGTCTTCTGCTT23229361.9086152517070294Illumina Single End Adapter 2 (95% over 24bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTG20681131.6992426885861598No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGTATGCCGTCTTCTGCT19829731.6292883280138768No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTGCTT13410941.1018953868607602TruSeq Adapter, Index 15 (95% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGATCGTATGCCGTCTTCTGCTTGA12760541.0484559739922932Illumina Single End Adapter 1 (95% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTCGTATGCCGTCTTCTGC10399630.8544743561643529No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATATCGTATGCCGTCTTC8456740.6948388997252143No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGCTT8396060.6898531931248783TruSeq Adapter, Index 21 (95% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGCCGTCTTCTGCTTG7319980.6014382432486483Illumina Single End Adapter 2 (96% over 25bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCGTATGCCGTCTTCTG6607420.5428915211798371No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTCGTATGCCGTCTTCT5933430.4875138614639421No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTCGTATGCCGTCTTCTG5423660.44562916051382157No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTCGTATGCCGTCTTCTG5406920.44425373651102623No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGTATGCCGTCTTCTGC4766580.3916408926669393No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTCGTATGCCGTCTTCTGC4467780.36709031369231143Illumina Single End Adapter 2 (95% over 21bp)
CTTTGGTGACTCTAGATACCCTCGGGCCGATCGCACTCGTATGCCGTCTT4434260.36433617912996363No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCTCGTATGCCGTCTTCTGCTTGAAA4282960.35190477819669325Illumina Single End Adapter 2 (96% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGCTTG3975900.3266755252517494TruSeq Adapter, Index 14 (95% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTTTGCT3793800.31171347561560575Illumina Single End Adapter 2 (95% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTGCTT3772560.3099683139776503Illumina Single End Adapter 2 (95% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCTCGTATGCCGTCTTCTGCTTGA3432060.28199149958387265RNA PCR Primer, Index 26 (96% over 29bp)
CTCCCGGGGCTACGCCTGTCTGAGCTCGTATGCCGTCTTCTGCTTGAAAA3295210.2707473672790606Illumina Single End Adapter 2 (96% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATCGTATGCCGTCTTCT3049990.25059913108040527No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTGC2873010.2360577607091548Illumina Single End Adapter 1 (95% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGTATGCCGTCTTCTGCTT2607790.21426624613201029Illumina Single End Adapter 2 (95% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTCGTATGCCGTCTTCTGC2529780.20785663881671335No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTCGTATGCCGTCTTCTGCT2459710.20209941301767664TruSeq Adapter, Index 16 (95% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATCGTATGCCGTCTTC2396380.19689597203219078No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTTCTTG2367100.19449021248608267Illumina Single End Adapter 1 (95% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCTCGTATGCCGTCTTCTGCTTG2319280.19056113388311513Illumina PCR Primer Index 3 (96% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTCGTATGCCGTCTTCTGC2312870.19003446316280936No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCGTATGCCGTCTTCTG2294200.18850046279648974No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTTC2280550.18737892530317088No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGATCGTATGCCGTCTTCTGCTTGAA2262840.18592380229901875Illumina Single End Adapter 1 (95% over 23bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTCGTATGCCGTCTTCTGCTTG2208440.1814540851095283TruSeq Adapter, Index 16 (96% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTCGTATGCCGTCTTCT2070520.17012203741146714No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTTCTT2047080.16819611515187788Illumina Single End Adapter 2 (95% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTCGTATGCCGTCTTCTGCT2030890.16686588130449093Illumina Single End Adapter 1 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTTTGCTT1962030.16120807384735278Illumina Single End Adapter 2 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTTCGTATGCCGTCTTCTGCTTGA1936150.15908167162558784Illumina Single End Adapter 1 (95% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTTCT1744200.14331030738803827Illumina Single End Adapter 2 (95% over 21bp)
CTTTGGTGACTCTAGCTAACCTCGGGCCGATCGCACTCGTATGCCGTCTT1707610.14030392959459237No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTTCGTATGCCGTCTTCTGCTTGAA1554460.12772052541131174Illumina Single End Adapter 1 (95% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGCCGTCTTCTGCT1538790.1264330168017655Illumina Single End Adapter 2 (95% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTCGTATGCCGTCTTCTG1427650.11730131885250134No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTCGTATGCCGTCTTCTG1399400.11498018814288541No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGATCGTATGCCGTCTTCTGCTTG1374880.11296552885085773Illumina Single End Adapter 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)