FastQCFastQC Report
Mon 3 Jul 2023
SRR5350741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5350741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138030486
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGC2533157518.35215953669829TruSeq Adapter, Index 21 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTGCTTG1751248012.687400086383816Illumina Single End Adapter 2 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGCT132399809.592069392554338TruSeq Adapter, Index 14 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTGCTTGA35012652.5365881853085703Illumina Single End Adapter 2 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGCCGTCTTCTGCTT29549552.1407988087501195Illumina Single End Adapter 2 (95% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGTATGCCGTCTTCTGCT25299051.832859590163292No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTG23640801.712723086405709No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTCGTATGCCGTCTTCTG21869741.584413750452201No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTGCT18803161.362246887980964TruSeq Adapter, Index 15 (95% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTCGTATGCCGTCTTCTGC17500241.2678532480136309Illumina Single End Adapter 2 (95% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTTTGCT15005451.0871112922112003Illumina Single End Adapter 2 (95% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGCTTG12198250.883735930626224TruSeq Adapter, Index 14 (95% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTCGTATGCCGTCTTCTGC11165770.8089350638090197No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGCCGTCTTCTGCTTG10371160.7513673464860509Illumina Single End Adapter 2 (96% over 25bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTCGTATGCCGTCTTCTGCT10060220.7288404389157914TruSeq Adapter, Index 16 (95% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTTCTTG8801820.6376721733776987Illumina Single End Adapter 1 (95% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGATCGTATGCCGTCTTCTGCTTGA8401630.608679302918632Illumina Single End Adapter 1 (95% over 23bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCGTATGCCGTCTTCT7625110.552422165636655No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTGCTT7448130.5396003604595002TruSeq Adapter, Index 21 (95% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTCGTATGCCGTCTTCT6848710.4961737220862933No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTTTGCTT6739120.4882341716887094Illumina Single End Adapter 2 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGTATGCCGTCTTCTGC6412490.46457055870976216No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTCTTC5520780.3999681635548251No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTCGTATGCCGTCTTCTG5478570.3969101434591776No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTTTTCT5363680.38858661991525556No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTGCTT5186930.37578147772369647TruSeq Adapter, Index 15 (95% over 24bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTGCTT4940710.35794338940456966Illumina Single End Adapter 2 (95% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTCGTATGCCGTCTTCTGCT4472170.3239987143130105Illumina Single End Adapter 2 (95% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCGTATGCCGTCTTCTG4340430.3144544459547871No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCTCGTATGCCGTCTTCTGCTTGAAA4229400.30641057077782075Illumina Single End Adapter 2 (96% over 26bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCTCGTATGCCGTCTTCTGCTTG4217030.3055143919438203Illumina PCR Primer Index 3 (96% over 26bp)
CGGCACTGGCAATTTTTGACAGTCTCTACGGAGATCGTATGCCGTCTTCT4095840.29673444749010014No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTCGTATGCCGTCTTC3928500.2846110387526999No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTTTTCTT3800910.2753674286128356No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTCGTATGCCGTCTTCTGCTTG3762390.27257674076435545TruSeq Adapter, Index 16 (96% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCTCGTATGCCGTCTTCTGCTTGA3718150.2693716517088841RNA PCR Primer, Index 26 (96% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTTTGC3597480.26062938009216313No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGTCGTATGCCGTCTTTTGCTTG3148230.22808222235774783Illumina Single End Adapter 1 (95% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCGTATGCCGTCTTCTGCTT3058290.22156627051215338Illumina Single End Adapter 2 (95% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTCGTATGCCGTCTTCTGC2991620.21673617812227366No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTTCGTATGCCGTCTTCTGCTTGA2861330.2072969590210673Illumina Single End Adapter 1 (95% over 23bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCTCGTATGCCGTCTTCTGCTT2856290.20693182229322876TruSeq Adapter, Index 16 (95% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTCTTCT2734730.19812507216702838Illumina Single End Adapter 2 (95% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCGTCTTTTGCT2641190.19134830837297784No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCTTCTTCTTC2558720.18537354132042974No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTCGTATGCCGTCTTCTGCTTG2537490.18383547530217345Illumina Single End Adapter 1 (95% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGCCGTCTTCTGCT2076620.1504464745563527Illumina Single End Adapter 2 (95% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCTCGTATGCCGTCTTCTGCTTGAAAA2011650.14573954336435505Illumina Single End Adapter 2 (96% over 26bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGATCGTATGCCGTCTTCTGCTTG1972330.14289089730510693Illumina Single End Adapter 1 (95% over 23bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTCGTATGCCGTCTTCTGCTT1834030.13287137161858575TruSeq Adapter, Index 19 (95% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTCGTATGCCTTCTTCTGC1811940.13127100052375387No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCGTATGCCGTCTTTTGCTT1810510.13116740022200604No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCGTATGCCGTCTTCTG1809770.1311137888770456No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTCGTATGCCGTCTTCT1807230.13092977155785715No Hit
AAGTCCTTCTGATCGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1740380.12608663857055463Illumina Single End Adapter 2 (95% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTCGTATGCCGTCTTCTGC1710550.12392552178654213TruSeq Adapter, Index 21 (95% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTCGTATGCCGTCTTCTG1562700.11321411995897776No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTCGTATGCCGTCTTTTGC1503930.1089563649004322No Hit
GGGGCTCTCGCTTCTGGCGCCCAGCGCCCGGCCTCGTATGCCGTCTTCTG1488090.10780879232722546TruSeq Adapter, Index 21 (95% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTTCGTATGCCGTCTTCTGCTTGAA1475750.10691478692612877Illumina Single End Adapter 1 (95% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTCGTATGCCGTCTTCTGCT1460330.10579764241357521Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGTCGTATGCCGTCTTCTGCTT1435360.10398862175997844Illumina Single End Adapter 1 (95% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGATCGTATGCCGTCTTCTGCTT1434720.10394225519136403Illumina Single End Adapter 1 (95% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTCGTATGCCGTCTTCT1420120.1028845178448477TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)