FastQCFastQC Report
Mon 3 Jul 2023
SRR5382420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5382420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16584922
Total Bases526.4 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT12715717.667030330320516No Hit
ATGTACACGGAGTCGACCCGCAACGCGA10526046.346752791481322No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8889825.360181977340623No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3821902.304442553302331No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2901741.749625352473771No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2636071.5894376832161166No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC2337921.409665960442865No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT1862611.1230743201565856No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1624070.9792448827917309No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1334120.8044174099823924No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1252660.7553005072921054No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1197980.7223308014351831No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1149410.6930451647586887No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1036500.6249652545848573No Hit
TACACGGAGTCGACCCGCAACGCGA1029730.6208832335780656No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA996010.6005515129947552No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT932550.5622878419325699No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT853200.5144431791720214No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA828830.4997491094621971No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC782130.47159100296039985No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC698250.42101494357344577No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT655920.3954917605280266No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA573990.34609146790078366No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC492870.2971795706968052No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC490100.2955093789407029No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG471580.28434260951001156No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG457870.2760760647532741No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG455150.2744360208628054No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG442220.2666397828099523No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTAT433360.26129758101967554No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT424260.2558106694743575No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC408430.2462658552147547No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC407890.24594025826591165No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT402780.2428591463981561No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC400780.24165323177281145No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT365170.22018192186855023No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG354630.21382675179298402No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT343530.2071339256223213No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT341050.20563859148689392No Hit
AAATTTTTGACAGTCTCTACGGAGACTG326320.1967570302712307No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT321200.19366988883034844No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC314170.18943109892226204No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT289030.17427275208167997No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC288760.17410995360725845No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA274030.1652283923915952No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT270670.16320245582101622No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT262300.15815570311394891No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG256890.1548937040523917No Hit
TATGTACACGGAGTCGACCCGCAACGCGA235820.14218939347438597No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC234060.1411281886040827No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC233470.14077244378960602No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT228880.13800486972444007No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTAT223910.13500817188045866No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG218940.13201147403647723No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC215660.13003377405091204No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC210860.1271395789500849No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT203070.12244254148436753No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC192970.11635267262637713No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC190990.11515881714728594No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTT190520.11487542721032996No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA187790.11322935374673453No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTATT186820.11264448515344239No Hit
TGTACACGGAGTCGACCCGCAACGCGA186650.1125419824102881No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT185000.11154710284437877No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC184530.11126371290742278No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC179160.10802583213837243No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA173980.10490251325872982No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC172760.1041669053372696No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC170620.10287657668815084No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC169650.1022917080948587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)