FastQCFastQC Report
Mon 3 Jul 2023
SRR5382421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5382421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24643006
Total Bases789.2 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT24255799.842869818722603No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT22900699.292977488217144No Hit
ATGTACACGGAGTCGACCCGCAACGCGA8744213.5483536383507754No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT7473573.0327347240024207No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3637621.476126735512705No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC3440581.3961689576344705No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2459480.9980438263091767No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2087040.8469096667833461No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2070210.8400801428202388No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2041300.8283486194825421No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA1854610.752590816234026No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1772740.7193684082209776No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1641880.6662661202939285No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT1557060.6318466180627478No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA1461900.5932311991483507No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1435050.5823356127900955No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1370030.5559508446331588No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1361840.5526273864479033No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT1317660.5346993788014336No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1153150.4679421008946717No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1041910.42280150400482797No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1035240.4200948536878983No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1009190.4095239030498146No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTT969780.3935315358848673No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG965040.3916080692428513No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA954530.3873431674690985No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT942250.3823600091644664No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC888900.3607108645755311No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG802230.32554064224145385No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC792560.3216166079738811No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC769780.31237260584199833No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT732020.2970497998499047No Hit
TACACGGAGTCGACCCGCAACGCGA655940.2661769428615973No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT630040.2556668614210458No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT617170.2504442842727872No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC590160.23948377077049773No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA515340.20912221504146045No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT506450.2055147006010549No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC493400.20021908041575773No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG485360.19695649142803437No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT474440.19252521384769375No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT463900.18824813823443454No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT432730.1755995189872534No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG398150.1615671399828414No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG394030.15989526602395826No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC377840.15332545063698805No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC353350.14338753965323872No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT347430.1409852353239698No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTAT347250.14091219228693125No Hit
TATAAACGATGCCGACCGGCGATGCGGCGGCGTT346030.14041712281366972No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG345840.140340021830129No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT326320.13241891025794497No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG325010.1318873192661642No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC322590.1309052962126455No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC318390.12920095868174525No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGGGA308900.1253499674512111No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC306030.12418533680509593No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC305590.12400678715900161No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA304580.12359693456228515No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT301650.12240795623715711No Hit
AAATTTTTGACAGTCTCTACGGAGACTG298870.12127984710956123No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTAT276370.11214946747973846No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT273430.11095643120810829No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT268140.10880977750847441No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC264950.10751529257429066No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA257770.10460168698575166No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG251130.1019072105083284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)