FastQCFastQC Report
Mon 3 Jul 2023
SRR5382422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5382422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21695005
Total Bases694.3 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT301884313.91492189100671No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT267958312.351151797383777No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT7305233.3672405237979897No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT4978782.294896912906911No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT2863921.3200826641892915No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2022080.9320486443768969No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1968750.9074669491894563No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1501710.6921915897230722No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1450260.6684764534509211No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1390710.6410277388735334No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1360140.6269369377882144No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1316960.6070337388721505No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1232630.5681630402942982No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1165460.5372019964964285No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA1018600.46950899527333595No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT972820.44840736381485047No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC941930.434169063339695No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT936090.4314771994751787No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA920890.42447097845794457No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT915450.42196348883072393No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT858600.3957593003550817No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC836720.38567402957501046No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT833450.38416677018511863No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC784390.36155326998080894No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA772770.35619719838737074No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC772690.3561603235399116No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT735710.3391149253019301No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT718620.33123753601347405No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG706060.32544818496239114No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT663180.30568326672429896No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC638360.2942428453001048No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT561210.2586816642817091No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC540650.24920482848471343No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT505920.23319653533151988No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC486120.22407001058538592No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC448100.20654523933043575No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT447490.2062640686185599No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC435780.20086651282173015No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT411490.1896703872619527No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG400610.1846554080075114No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTT396570.1827932282108255No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT369590.17035718590523485No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGGGA359570.1657386112609792No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG356220.16419447702362824No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT345240.15913340420986305No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG343680.15841434468441007No Hit
ACGGGGTCGGCGGCGACGTCGGCTACCC298420.13755239973440891No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT294980.13596678129366646No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA291660.13443647512411266No Hit
AAATTTTTGACAGTCTCTACGGAGACTG290740.13401241437833272No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGC287980.13274023214099284No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG287380.13246367078504936No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT285050.1313896908528023No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTAT272790.12573862047969106No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC262300.12090340610661302No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGC255650.11783818441157307No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG243370.11217789532659707No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTT229470.10577089058057373No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC218300.10062224000409312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)