FastQCFastQC Report
Mon 3 Jul 2023
SRR5382423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5382423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8197726
Total Bases250.8 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT101637012.398194328524765No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT92489511.282336101499366No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2134122.6033073074167152No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1017171.2407953132368659No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT990521.2082862979318898No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT805010.981991835296764No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT781540.9533619445197363No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC740270.9030187151900417No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT597590.7289704486341699No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG594450.7251401181254411No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC470490.5739274525642842No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG447350.5457001124458173No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA413830.5048107243398962No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA342650.4179817671388383No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT318550.3885833705591038No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTT277540.3385573023543358No Hit
ACGGGGTCGGCGGCGACGTCGGCTACCC275250.3357638447540208No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC267480.32628560652063754No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG221950.27074581414406873No Hit
ACTGGCAATTTTTGACAGTCTCTACGGAGACTG220230.26864767131763123No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG218560.2666105210152181No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC214780.2619994861989776No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG207680.2533385477875206No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT178150.21731636309874225No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG175840.2144985084888175No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC167400.20420297043350802No Hit
TCGACTGCATAATTTGTGGTAGTGGGG152860.18646634444722843No Hit
TCGACTGCATAATTTGTGGTAGTAGGGG141810.1729869966378481No Hit
CTGGCAATTTTTGACAGTCTCTACGGAGACTG137900.16821738125914432No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA132480.16160579165490527No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG130310.15895871611224868No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC127220.15518937812754413No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT126530.15434768129600818No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC120990.14758970963411072No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC116280.1418442138710174No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC115920.14140506769804212No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT115550.14095372302026196No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT112040.13667204783375292No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA104420.1273767871724427No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC102570.12512006378354193No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA102410.12492488770666402No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT93650.11423899749759872No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGC92220.11249461131050244No Hit
ACGGGGTCGGCGGCGACGTCGGCTACCCACCC90860.11083561465704025No Hit
ACGGGCCCCTCTCTCGGGGCGAACCC90450.11033547596004062No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTC88260.10766400340777431No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGGGA86750.10582202918223908No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT84360.10290658653387538No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA83130.10140617044287648No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC83060.10132078090924239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)