FastQCFastQC Report
Mon 3 Jul 2023
SRR5382424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5382424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6868226
Total Bases210.2 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT87094912.680843641429387No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT79571211.585407935033006No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1770532.5778563489320243No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG876561.2762538681749842No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT805641.1729957633892654No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT793301.1550289696349536No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT662200.9641499857459555No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC604030.8794556265329649No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT520020.7571387429592444No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC412280.600271452919575No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG392320.5712100912229737No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA301420.43886150513975514No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA298230.4342169287964607No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG284270.41389144736937894No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT225000.3275955101069767No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG198100.2884296468986315No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC193810.2821834925059251No Hit
ACGGGGTCGGCGGCGACGTCGGCTACCC192860.28080031146325124No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTT185600.2702298963371328No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC173970.2532968484147144No Hit
ACTGGCAATTTTTGACAGTCTCTACGGAGACTG169520.24681773721482084No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG157450.2292440580726377No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG147630.2149463340315243No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT146070.21267500516144927No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG135600.19743089409113793No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC132790.19333959016491303No Hit
TCGACTGCATAATTTGTGGTAGTGGGG126700.18447267169135087No Hit
TCGACTGCATAATTTGTGGTAGTAGGGG121120.17634830304069785No Hit
CTGGCAATTTTTGACAGTCTCTACGGAGACTG111240.16196322019688927No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT107480.15648873522799045No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA104460.15209167549233238No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC101410.1476509363553267No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT94100.13700772222696225No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC90400.13162059606075863No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC87860.12792240674666208No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGGGA85400.12434069583615916No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT82570.12022027230903586No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC82350.11989995669915347No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT82320.11985627729780587No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG80220.11679871920347408No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC80220.11679871920347408No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGC79980.11644928399269332No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTC76800.11181926744984803No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA75980.11062536381301373No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA75150.10941690037573021No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA74050.10781532232631832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)