FastQCFastQC Report
Mon 3 Jul 2023
SRR5398651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6633366
Total Bases338.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1803452.7187554553751445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1050641.5838715970142458No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT982121.4805756232959255Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC969591.4616862690826948No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG795791.1996775091258345TruSeq Adapter, Index 12 (100% over 49bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG701531.0575777064012448No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT687531.0364722826993114Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG414760.625263252472425Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG412840.6223687943647312No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC402470.6067357055226562Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT379490.5720926600461967No Hit
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA376510.5676002198582139Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG339710.5121231061274171No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC302470.4559826790802739No Hit
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA274570.41392258470284926Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC263420.39711362225452357No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG262570.39583222152976333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC247160.3726011801549922No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC241940.3647318721746999No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT233010.3512696269133951Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT219690.33118932379126975No Hit
ACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC219080.33026973032997126Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC218420.32927476035545156No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT209380.3156466867650602No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA208010.31358137030279953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT202860.30581758944101683No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT197390.2975713988946185No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC192660.2904407807438938No Hit
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC175090.26395347399796726Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGA173230.26114946770613895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT169620.2557072834515689No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT165640.2497073129991621No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT163450.24640582172007394Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG163050.24580280961430442No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC158620.23912445054290687No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC151690.22867726581044978No Hit
CAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAG141350.21308940287630743Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG140900.2124110142573167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA135970.20497889005370729Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT127900.192813120819807No Hit
AGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAAC126050.19002418983062294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG120630.18185337579744582No Hit
AACCCGGCGGCGCGCAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTC116590.17576295352917357No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG115430.17401421842244194No Hit
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG113150.17057704941955562Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC111310.16780319373301578No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC109260.16471275669094695No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC107030.16135096420128184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG103990.15676807219743338Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG102120.15394899060296086No Hit
CAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC101330.15275804169406604Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA100880.15207965307507532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC100790.15194397535127718Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG96620.14565757414862981No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT95100.14336612814670563No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC94890.14304954679117662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA92200.1389942903798765No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA90800.13688374800968317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT90070.13578325091665377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA89110.1343360218628069No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT88400.133265675375066No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG87850.1324365337296329No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC85220.12847172913419824Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC85120.12832097610775586Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCA82710.12468782817049444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT82000.12361748168275351Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC81860.12340642744573418Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCAC77990.117572285322414No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA77850.11736123108539466No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA75320.11354717951640239Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAACT75200.11336627588467152Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA73560.11089392625101646Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT73400.11065272140870863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT72800.10974820325005434No Hit
AGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC72120.10872308267024615Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT71040.10709494998466841No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC70960.10697434756351451Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC68950.10394421173202262No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC68930.10391406112673415No Hit
CAGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTC68590.10340150083683006Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC68490.10325074781038766Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCGGGCGTGGAATGCGAGTGCCTAGTGAGATCGGAAGAGCACACGTCT66950.10092915120317497Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC66520.10028091318947273Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAGATCGGAAGAGCA66460.1001904613736073No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)