FastQCFastQC Report
Mon 3 Jul 2023
SRR5398653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16170310
Total Bases824.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2860231.7688158111996617No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2100481.2989732417003754No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1511910.9349913514335841No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1499840.9275270542123187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1363730.8433542708828712No Hit
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1346880.8329339388051311Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGA1225290.7577405751652256Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1145100.7081496891525271Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1121530.6935735925903709Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1111030.6870802105834706TruSeq Adapter, Index 7 (100% over 49bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1033030.6388436585322111No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1019820.6306743655501966No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG924510.5717330094475617No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT899330.5561612609776807No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT886790.5484063076094398No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG859640.5316162769915975No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT853930.5280851140145119No Hit
GTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAACT814070.5034349990816502Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC756410.46777705560375776No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA670400.4145869807072345No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT650360.4021938973340647No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT646390.39973878051812245No Hit
AGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAAC573090.3544087899366184Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT571370.35334511212215475No Hit
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG509580.31513310505488146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC501900.31038365992983435No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA485090.299988064545454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA464120.2870198530516731Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA458870.28377316204822295No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA449950.27825687942902766Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA446200.2759378144265633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG432340.26736655017745486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC421990.2609659307706531No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC418220.2586344974215089No Hit
TCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAACTC414370.25625359068564546Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA404680.25026112671927747No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG390520.24150433726997195No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC385360.23831330382658092No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT381470.2359076603973579No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC378890.23431214367566236No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT376000.23252491758042979No Hit
GACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAACT372310.23024295761800484Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG366290.22652008526738202No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA363980.22509154122586394Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT350790.2169346165905292No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA350140.21653264532343536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC349800.2163223834298786No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC348030.2152277847487154Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG346990.21458463072136527No Hit
TGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTGAACTCC344580.21309424494644816Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT341110.21094833679750108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA337980.20901269054211083Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA331050.20472705841755665Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG321080.1985614375976713Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC310090.19176503109711565No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA307610.1902313561088192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC292350.1807943075921241Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC285060.17628604522733332No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG274590.16981121574045271No Hit
GCACGAGCGTACGCGTTAGGACCCGAAGATCGGAAGAGCACACGTCTGAA274110.16951437542013725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC257060.15897035987559915Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG250920.15517327744489748No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT248570.1537199967100198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT243170.15038054310647106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG242220.14979304663918008Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTG232900.14402939708638857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT230210.1423658544579541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG230180.1423473019379344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGTACGCAGTTTTATCCGGTAAGATCGGAAGAGCACACGTCTGAAC226680.14018284126896763Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC218690.1352416867703835Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA215360.13318235704819512No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC212450.13138276260628276No Hit
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC211190.1306035567654547Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGAA210640.13026342723175993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT208290.12881014649688224No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC207820.12851949034990673No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG204970.12675700094803377No Hit
ACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA200720.12412872727857413Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC197800.12232294866332184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC197220.12196426660960737Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA195410.12084493123508454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC194310.120164672167695Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT193650.11975651672726127No Hit
GCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCAC190730.11795073811200898No Hit
GACGTACGCAGTTTTATCCGGTAAGATCGGAAGAGCACACGTCTGAACTC189300.11706640132440255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA188730.11671390344402799Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG188620.11664587753728903No Hit
GAGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAA188270.11642943147039235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA187640.11603982854997832No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG186940.11560693641618498No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG186250.11518022845573152Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC185160.11450615356168187No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCT181750.11239735045277426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG178320.11027617899718681Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT177710.10989894442345262Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT177030.10947842063633907No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC175050.10825395431503786No Hit
AGCTGGTTCCCTCCGAAGTTTCCCAGATCGGAAGAGCACACGTCTGAACT174950.10819211258163881Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC174860.10813645502157967No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA173240.10713461894051506Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT171060.10578646915241575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAAC165660.10244701554886704Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA165150.10213162270853186Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG162340.10039387000001854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)