FastQCFastQC Report
Mon 3 Jul 2023
SRR5398655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379180
Total Bases172.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG841492.490219520712125TruSeq Adapter, Index 8 (100% over 49bp)
AACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGA534131.580649743428879Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC519611.5376807391142229No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA407761.2066832781917507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG329530.9751774099041779Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT253180.7492350215140952No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT238140.7047271823341758No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC234800.6948431276226777No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG231960.6864387218200865No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT228720.6768505968903699No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT209110.6188187666830414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC201780.5971271136784664No Hit
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG199750.5911197391083044Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT196080.5802591161169278No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG191840.5677116933693973No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG170000.5030806290283442No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC164940.4881065820702064Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC154910.45842482495753406No Hit
AGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAAC143650.4251031315289508Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGAA141690.41930290780603574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA139550.41297001047591425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT134940.3993276475357927Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG134920.3992684615794364No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA128040.37890849259287757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA123720.366124326019922No Hit
TCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAACTC119710.3542575417704887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT116400.3444622659935251No Hit
GTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAACT113980.33730076527441566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA110200.3261146195230795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG108110.3199296870838488Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG107680.3186571890221888No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA107440.3179469575459135Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC106520.3152244035535248No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT103660.30676081179457737No Hit
AGGCGGGGGACCGGCTATCCGAGGCCAGATCGGAAGAGCACACGTCTGAA103620.3066424398818648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC102860.30439337354032636No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT99510.2944797258506502No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC93660.2771678336164395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCT93320.2761616723583828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC87290.25831710651696566No Hit
GCACGAGCGTACGCGTTAGGACCCGAAGATCGGAAGAGCACACGTCTGAA85280.25236891790315996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG82990.2455921259003664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT81380.24082765641368617No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA79990.23671423244692502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC75160.2224208239868844No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA73590.21777472641291676No Hit
CTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCC71830.21256636225356448Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT70430.20842334530862516No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC67640.2001669043969247No Hit
TGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTGAACTCC66740.1975035363608923Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT64410.19060837244538614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGTTCCCTCCGAAGTTTCCCAGATCGGAAGAGCACACGTCTGAACT64030.18948383927461693Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT62230.1841571032025521No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTG61460.1818784438828355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT61090.18078350369024437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCTCCCGACGTACGCAGATCGGAAGAGCACAC58860.17418426955651964No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA58730.17379956084020384Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC58570.17332607318935364No Hit
ACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA58460.1730005504293941Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGGGCGTGGAATGCGAGTGCCTAGTGAGATCGGAAGAGCACACGTCT58110.1719647961931593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC55250.16350120443421184No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC52250.15462331098077048No Hit
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG49980.1479057049343332Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC49160.14547908072372587No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC48660.14399943181481897No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG48460.14340757225125622No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAA47290.1399451938044141Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC46790.1384655448955072Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT46720.13825839404826024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG46530.1376961274628756No Hit
GACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAACT46470.13751856959380676Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT45140.13358270349611442No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA45070.13337555264886747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACG44670.13219183352174196No Hit
GTCTAACGCGTGCGCGAGTCAGGAGATCGGAAGAGCACACGTCTGAACTC44560.13186631076178246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGATTCCCTCAGTAACGGCGAGTGAACAGATCGGAAGAGCACACGTCTG44350.13124485822004153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG44330.13118567226368527No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA44280.13103770737279458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGTCTAACGCGTGCGCGAGTCAGGAGATCGGAAGAGCACACGTCTGA43990.13017951100562858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT43440.128551897205831No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC43060.12742736403506177No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC42710.12639160979882694No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA42160.12476399599902935Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC42140.12470481004267307Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA41520.12287004539562853No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA41230.12201184902846253No Hit
AGTCTAACGCGTGCGCGAGTCAGGAGATCGGAAGAGCACACGTCTGAACT40210.11899336525429247Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA40140.11878621440704551No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC39980.11831272675619528No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG39900.11807598293077019No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT39540.11701063571635721Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTG39310.11632999721826005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCATCTAAGGCTAAATACCGGCACGAGAGATCGGAAGAGCACACGTCTG38270.11325232748773371Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG38150.11289721174959606No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT38110.11277883983688351No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA36840.10902053160825999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCATCTAAGGCTAAATACCGAGATCGGAAGAGCACACGTCTGAACTC36510.10804396332838144Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA36140.10694902313579033Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGGTTCCCTCCGAAGTTTCCAGATCGGAAGAGCACACGTCTGAACTC35910.10626838463769317Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC34910.10330908681987938No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA34760.10286519214720731Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA34630.10248048343089151Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG34510.10212536769275388No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG34410.10182943791097249Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTC34150.1010600204783409No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCAGATCGGAAGAGCACACGTCTG34080.10085286963109394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA33820.10008345219846235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)