FastQCFastQC Report
Mon 3 Jul 2023
SRR5398657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13031785
Total Bases664.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2734672.098461569155722TruSeq Adapter, Index 9 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2457441.885727856928272No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1897051.455710019770891Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1671961.282986175723433Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1447281.110576947056754No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1435201.1013073036425938No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1361791.044975803391477No Hit
AACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGA1068300.8197649055750997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG822950.6314944575896548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC723550.5552194116155231Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA654790.5024561101951881Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT623330.4783151348798342Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT622220.4774633712879702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG603380.4630064108638993No Hit
CAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC585090.44897149546282417Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC533500.4093836723058276Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG524220.4022626217360093No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC486670.3734484569842121No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC486660.3734407834383394No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT483720.3711847609517806Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT474950.36445506122146737No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT468980.3598739543354959Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT467960.35909125265648567No Hit
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG465010.3568275566240542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC462190.35466361668796714No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT455580.34959140286614615No Hit
GTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAACT389320.2987464879139734Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC358880.27538821427762966No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA354680.27216532501111707Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT337730.2591586647569769No Hit
ACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC333270.25573626329777543Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTGAAC332910.25546001564636006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG324670.2491370138472972Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA312010.2394223047725235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA301150.2310888339548266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT282360.21667024126011902No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC279500.2144756071405414No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT274650.2107539373923066No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT274180.21039328073629207No Hit
GACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAACT267680.20540547591907016Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT249020.19108663932070702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC248790.19091014776563608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG243090.18653622661822614No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC241060.18497849680607836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC240830.18480200525100743Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC240310.1844029808656297Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG236560.18152540116338628No Hit
TGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTGAACTCC235570.18076572012199402Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG230770.17708241810312247No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG230560.17692127363979684No Hit
AACCCGGCGGCGCGCAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTC228210.1751179903597243No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA221350.16985393789108705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT216060.1657946321244557No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC209190.1605229061099458Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG205840.15795226824260836Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC204430.15687029827456483No Hit
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG193060.14814547661736285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT185320.1422061521119325No Hit
AGCTGGTTCCCTCCGAAGTTTCCCAGATCGGAAGAGCACACGTCTGAACT184670.1417073716302103Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT173530.13315904152807923No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA166410.1276954768667531Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC166360.12765710913738984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC165870.12728110538963003No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGAGATCGGAAGAGCACACGTCTG164320.12609170577936943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG163220.12524761573337806No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG161640.1240351954854995No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC161200.12369755946710294No Hit
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA157360.12075091785200569TruSeq Adapter, Index 9 (100% over 35bp)
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC149530.11474253143372148No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC149270.11454301924103259No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCT147940.11352243763997026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCTCCCGACGTACGCAGATCGGAAGAGCACAC147930.11351476409409762No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC146490.11240977348843616No Hit
TGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG146370.11231769093796437Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA145040.11129710933690204Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAG143560.11016142454774998Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC142580.10940941705223038No Hit
CGACGAAGCCGAGCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC142510.10935570223112184Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG140950.10815862907498858No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAGATCGGAAGAGCA132740.1018586479135437No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG132450.10163611508323686No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT131540.10093782240882582No Hit
GCACGAGCGTACGCGTTAGGACCCGAAGATCGGAAGAGCACACGTCTGAA130950.10048508320233951Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)