FastQCFastQC Report
Mon 3 Jul 2023
SRR5398659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16386558
Total Bases835.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4494632.7428762037762904No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC4233132.5832941853926856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT2533501.5460842966533912No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2340901.4285489362683732No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC1514080.9239768351596473No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1256220.76661614965144No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC1229820.7505053837419671No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG1019900.6224003845102797No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1006350.6141314118559859No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA987960.6029087987849553No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG912720.5569931159429576TruSeq Adapter, Index 9 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT909790.5552050650295199No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA761650.4648016990511369Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA629070.3838939208587917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT586440.3578786954526997No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC557270.3400775196353011Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC542540.3310884445653566No Hit
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC525550.32072019029255566No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTC500330.30532952679873343No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA496080.3027359375898221No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC487330.29739619510088694No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC468520.2859172743903875No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC456370.2785026605343233No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC413080.2520846659804945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGA378430.23093928572431138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCTGAACT371760.22686887630703167Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGATCGGAAGAGCACA367310.22415323584123034No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT365270.2229083130209529No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACA362680.2213277492442281No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG360730.22013774948955114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAA340660.20788990586064504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA328150.20025559974217894No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCT323000.1971127798772628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCAC311280.18996057622351198No Hit
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACT302400.18454150041759837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT302250.18444996197493094No Hit
ACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACACGTCTGAACTC301530.1840105774501271Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTC293640.17919565536581875No Hit
TTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAAC284110.1733799129750128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG282750.17254996442816117No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA268940.1641223251399104No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT267510.16324965865314728No Hit
GTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACT260640.15905719797897766Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACG256560.15656735233842276No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA250050.152594583926655No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC249870.15248473779545405No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG241030.14709007224091844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA238030.14525930338756926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGT236410.1442706882067607No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCAGATCGGAAGAGCACACGTCTGAA234770.14326986790026314Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTC225290.1374846383236797No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC215090.13126002422229244Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTCT213190.13010053728183796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT211610.12913633235240737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT210960.12873966576751505No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACG210790.12863592219915862No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGT205880.12563956384251043No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT205310.1252917177603741Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAA201420.12291782081386464Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTC197530.12054392386735518No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGAA197290.12039746235908726Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC196560.11995197527143896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGATCGGAAGAGCAC196130.11968956506912555No Hit
ACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTGAA195380.11923187285578826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTCTG194870.11892064215071889Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTC189920.11589987354269274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCT188100.11478920710499424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGGGGGACCGGCTATCCGAGGCCAGATCGGAAGAGCACACGTCTGAA179180.10934572104770264Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAA178630.10901008009125528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTC174470.1064714139479444No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTC172620.1053424398217124No Hit
ACTCTGGACTCTGAATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACG172320.10515936293637748No Hit
GCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAAC172300.10514715781068848Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG171620.10473218353726267No Hit
ACAATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACG168540.10285259418115751No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA167540.10224233789670777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGT164990.10068618437136095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)