FastQCFastQC Report
Mon 3 Jul 2023
SRR5398661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4367689
Total Bases222.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG70905116.23400841955551TruSeq Adapter, Index 10 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT378580.8667741682157315No Hit
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA243170.5567475156770548Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT235030.5381106576040555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT220800.505530499080864No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT216370.49538783553499344No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC208140.47654491883465144No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC188560.4317157196860857No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGA151010.34574348127808546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC144310.33040356124257014No Hit
AGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCTGAACT135060.3092253134323437Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCAC115670.26483112694150157No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT114650.26249579583161714No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA112900.25848910029995265No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTC102720.2351815800071846No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC92680.21219459535694965TruSeq Adapter, Index 10 (100% over 50bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTC89050.20388356405412564No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT88190.20191455939285052No Hit
AAAGCACTGAAAATGCTTAGATGAGATCGGAAGAGCACACGTCTGAACTC86120.19717521096396742Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT83650.19152004641356102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTC76690.17558484589905554No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGT74160.16979230893042063No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA73400.16805225829952636Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA71530.1637708179314049No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC70360.16109205577594926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA68170.15607796251060915No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC67610.15479581994047653No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG64890.14856827031411807No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC62240.14250098850902618Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC62090.14215755746345493No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAA58470.13386942156366902Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACG58100.13302229165125995No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA54700.12523785461831188No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT52550.12031534296512413No Hit
AGCCCGACCGACCCAGCCCTTAGAGCCAGATCGGAAGAGCACACGTCTGA50950.1166520784790309Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTAGATCGGAAGAGCACACGTCTGAA50630.11591942558181227Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG49770.11395042092053716No Hit
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCT48340.11067637828609134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC48080.11008109780710121No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA47810.10946292192507297Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA46030.10538754018429425Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC45080.1032124768956764No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA45000.10302931367137175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGTCTGAAC44850.1026858826258005Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)