FastQCFastQC Report
Mon 3 Jul 2023
SRR5398663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18461508
Total Bases941.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC4511962.4439823659042372Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2489991.3487468087655679No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1534790.8313459550541592No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1052140.5699101070183432No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA1033230.5596671734508363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG922600.49974249124177716TruSeq Adapter, Index 11 (100% over 49bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG895290.4849495501667578No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT773390.4189202745517863No Hit
AGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCTGAACT750340.4064348372841482Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA697420.37776978998682015No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC694480.3761772873591908No Hit
GTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACT694230.37604187046908627Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCTGAA676410.366389354542435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC658520.35669892188655444No Hit
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC637300.34520473625448145No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT585560.3171788566784468No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT583170.3158842712090475Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT548050.2968609064871624No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC520770.28208421543895545No Hit
AGGCGGGGGACCGGCTATCCGAGGCCAGATCGGAAGAGCACACGTCTGAA502720.272307115973408Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT488950.2648483536664502No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG480430.26023334605168763No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG472540.2559595889999885No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTC446490.24184914905109592No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA426930.23125413156931707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAA410590.22240328363208467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC400150.21674827430131927Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC397810.2154807722099408No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA392250.21246910057401594No Hit
ACAATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACG390050.21127743194109602No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC377300.20437117054576473No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC376480.20392700314622184Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA352740.19106781526189517No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTC342580.18556447284804686No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC330420.17897779531336225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA328810.17810571054108906Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC327210.17723904244442001No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA323930.17546237284624852No Hit
ACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTGAA322960.17493695531264294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT322840.17487195520539275No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTC322640.1747636216933091No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC321890.1743573710229955No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC318310.17241820115669856Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC308450.1670773590109757Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA299370.162159017562379Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCTGGACTCTGAATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACG298360.16161193332635665No Hit
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCT292030.15818317766890982Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTCCCCGGACCCCGTCCCGGCCAGATCGGAAGAGCACACGTCTGAAC290330.15726234281619897Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA287120.15552358994725674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGGCTCGTGCGTCGATGAAGAACGCAGATCGGAAGAGCACACGTCTG287090.1555073399204442Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAACT284080.1538769205635856Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG282300.15291275230604132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT281690.15258233509418623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA276550.14979816383363698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT270460.1464994083906905No Hit
TTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAA268210.1452806563797497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT262880.142393568282721No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC255880.138601895359794No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTC247610.13412230463513597No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC244340.13235105171256867No Hit
AAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACT241680.1309102160018564Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG236410.1280556279584528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACT231600.12545020699284154Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT226870.12288811943206374No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT222700.12062936570512008No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA221090.11975728093284686No Hit
ACCACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACG220430.11939978034297091No Hit
ACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACACGTCTGAACTC218590.11840311203180152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAAC216360.11719519337206907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA215650.11681060940417218No Hit
ACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTGAACTCC205250.11117726677582353Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT204240.1106301825398012No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG203560.11026184859871685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG197710.10709309337027073No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC196050.10619392521997661No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC194390.10529475706968248Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCC193760.10495350650661905Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGCACACGTCTGAACT189250.10251058580913325Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA188990.10236975224342454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC188770.10225058538013254No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC186080.10079349964260775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)