FastQCFastQC Report
Mon 3 Jul 2023
SRR5398665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20111445
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC13744616.834223000883329Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC9901094.923112188109805No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA8776844.364102131895545No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG6220733.093129310201231TruSeq Adapter, Index 12 (100% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA5173862.572594858300833No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3458581.719707360659565No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA3104201.543499236380081Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTC1699640.8451108311710074No Hit
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC1672430.8315812215382834Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC1564980.778153931753785No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC1490690.7412147660200448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1392880.6925807668220757No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1021530.5079346610847704No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC982660.4886073576513274No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA972230.4834212559067735No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCT967920.48127819756362605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG966530.48058704881722825No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT937410.46610773119484955No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA852140.42370898759388No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC846690.4209990878328236No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGT817380.40642529664079335No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC792200.39390506251539853Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG786000.3908222407688756No Hit
TTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAA737500.366706619042043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAAC671140.3337104817679684Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT637070.31676987904151094Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAAC620850.3087048195691558Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC587320.2920327206722341No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAGATCGGAAGAGCAC577130.2869659539630295No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACA520360.25873824580978644No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT504860.2510311914434791No Hit
TCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAA461530.22948624526979539Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGCACTGAAAATGCTTAGATGAGATCGGAAGAGCACACGTCTGAACTC440940.2192482936954555Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC440920.21923834910917642No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT431670.21463897795508977Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA430180.21389810627729633No Hit
AGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAACT428640.21313237313380515Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT413560.20563415507935906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT410550.20413749484435353No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC401430.19960276350108108Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG396980.19739009305397995No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC395960.19688291915374553No Hit
AGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACACGTCTGAACTCC370220.1840842366125358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA317730.15798466992302146No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTC282630.14053192100318998No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG272570.13552979410479954No Hit
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT265400.13196465992373993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCTGGATACCGCAGCTAGGAATAGATCGGAAGAGCACACGTCTGAA255640.12711170181953607Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCT242880.1207670557734663Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC240300.11948420414346159No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC230680.11470085814321149No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAGATCGGAAGAGCACACGTC229610.11416882277727931No Hit
CTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTC229210.11396993105169718Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA226780.11276166381878577No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTC226300.11252299374808722No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT224660.1117075376732005No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC223210.11098655516796532No Hit
AGAGTGTTCAAAGCAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG218940.10886338599737612Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAA217880.10833632292458349Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCT217230.10801312387051254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTTTACTTTGAAAAAATTAGAGTAGATCGGAAGAGCACACGTCTGAAC216520.10766009105760425Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG216140.10747114391830126No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT215580.10719269550248628No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC209400.10411981834224243No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTAGATCGGAAGAGCACACGTCTG208700.10377175782247372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)