FastQCFastQC Report
Mon 3 Jul 2023
SRR5398667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12597525
Total Bases642.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG954456175.76536660971104TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC981560.7791689240545266TruSeq Adapter, Index 8 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC962890.764348552592672TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTTAACTCCAGTCACACTTGAATCTCGTATG821030.651739131297616TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTAACACTTGAATCTCGTATG761240.6042774275105626TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAATAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG722960.5738905062700808TruSeq Adapter, Index 8 (97% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC719290.570977235607788TruSeq Adapter, Index 8 (100% over 50bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG629860.4999871006408005TruSeq Adapter, Index 8 (97% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT628000.4985106201416548No Hit
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG607660.482364591457449TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTACAGTCACACTTGAATCTCGTATG447240.35502211743973516TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGTAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG439930.3492193903167487TruSeq Adapter, Index 8 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG404330.32095987108578866TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGCATCTCGTATG374490.2972726785618604TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG367130.2914302611028754TruSeq Adapter, Index 8 (97% over 49bp)
AGATCTGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG364530.2893663636309513TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTATGAACTCCAGTCACACTTGAATCTCGTATG356620.2830873524759824TruSeq Adapter, Index 8 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG355910.2825237497048031TruSeq Adapter, Index 8 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC352360.27970573584890684No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT350850.278507087701751No Hit
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG317480.25201775745632576TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTGAATCTCGTATG296850.2356415248233284TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCAAGTCACACTTGAATCTCGTATG277720.22045600227028722TruSeq Adapter, Index 8 (97% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT274620.21799520143837778TruSeq Adapter, Index 8 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTGAATCTCGTATG220210.17480417780476723TruSeq Adapter, Index 8 (97% over 49bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG217610.17274028033284317TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGTACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG190380.1511249233480386TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGATCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG186770.14825928108894407TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATTCGTATGC182720.14504436387306238TruSeq Adapter, Index 8 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATTGAATCTCGTATG172950.13728887221894778TruSeq Adapter, Index 8 (97% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC168830.13401838853266812TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACACTTGAATCTCGTATG147730.1172690667412845TruSeq Adapter, Index 8 (97% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGT144320.1145621858261841TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTGAATCTCGTATG143990.11430022960859376TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGAACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG143460.11387951204700925TruSeq Adapter, Index 8 (97% over 49bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG129380.10270271342982054TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)