FastQCFastQC Report
Mon 3 Jul 2023
SRR5398669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927198
Total Bases149.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG226578477.4045349853341TruSeq Adapter, Index 10 (100% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC289220.9880438562748404TruSeq Adapter, Index 10 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC224290.7662276347551481TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC221580.7569696344422209TruSeq Adapter, Index 10 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT215270.7354131835290951No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTTATCTCGTATG157850.5392528964559282TruSeq Adapter, Index 10 (97% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC130760.4467070556894341No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT120350.4111440360371933No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG118610.40519978491376396TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCTTATCTCGTATG113590.38805027879904264TruSeq Adapter, Index 10 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG98870.3377632807893419TruSeq Adapter, Index 10 (100% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGT94200.3218094573718621TruSeq Adapter, Index 10 (100% over 50bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG86840.29666595836701176TruSeq Adapter, Index 10 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC73270.25030763207681883TruSeq Adapter, Index 10 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT62890.214847099512913TruSeq Adapter, Index 10 (100% over 48bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA56930.19448633129702877No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG52830.18047976255791376No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTATCTCGTATG43780.1495628242435257TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATTCGTATGC41920.14320862476675647TruSeq Adapter, Index 10 (97% over 41bp)
AGATCGGAAGAGTACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG40300.1376743219966671TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATTATCTCGTATG39260.13412143626772086TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGTATGCC38310.13087601180377958TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTTAACTCCAGTCACTAGCTTATCTCGTATG36930.12616160574037014TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAATAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG33950.11598122163242802TruSeq Adapter, Index 10 (97% over 49bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG33890.11577624745575804TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCTTATCTCGTATG33320.11382899277739325TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTAACTAGCTTATCTCGTATG30730.10498094081780598TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTTATCTCGTATG30120.10289703668832789TruSeq Adapter, Index 10 (97% over 49bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG29890.10211130234442632TruSeq Adapter, Index 10 (97% over 49bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG29380.10036902184273151TruSeq Adapter, Index 10 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)