FastQCFastQC Report
Mon 3 Jul 2023
SRR5398671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13915683
Total Bases709.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1151981082.78292915985512TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1167800.8391970412088289TruSeq Adapter, Index 7 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1103920.7932920001123911TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC904270.6498207813443293TruSeq Adapter, Index 7 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT720720.5179192426271855No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG456520.32806151160528735TruSeq Adapter, Index 7 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG406540.29214520049069814TruSeq Adapter, Index 7 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATCATCTCGTATG333500.239657658197589TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTTCATCTCGTATG327540.23537472073774604TruSeq Adapter, Index 7 (97% over 49bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG317450.22812390883005887TruSeq Adapter, Index 7 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC308240.2215054769499995No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT303590.2181639233949207TruSeq Adapter, Index 7 (100% over 48bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT301410.2165973455992063No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC293260.2107406442069714TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCTCATCTCGTATG239800.17232355752858125TruSeq Adapter, Index 7 (97% over 49bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA207230.14891831036967426No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT194590.13983503360920194TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATCATCTCGTATG193130.13878585765427395TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGTATGCC182860.13140569528639018TruSeq Adapter, Index 7 (97% over 42bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG177500.12755392602720256No Hit
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCAGATCATCTCGTATGC162320.11664537055062264Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACCCAGTCACCAGATCATCTCGTATGC141460.10165508944117224Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)