FastQCFastQC Report
Mon 3 Jul 2023
SRR5398673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5398673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7076532
Total Bases360.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG618707387.43086302725686TruSeq Adapter, Index 9 (100% over 49bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC580820.8207692694670214TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC414920.5863324012383467TruSeq Adapter, Index 9 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC346800.4900705599861627TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCAGATCTCGTATG241030.3406046916766574TruSeq Adapter, Index 9 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG226510.3200861665007662TruSeq Adapter, Index 9 (100% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG198720.28081551810971817TruSeq Adapter, Index 9 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT176900.2499812054831378TruSeq Adapter, Index 9 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATG144920.2047895777197079TruSeq Adapter, Index 9 (97% over 49bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG138350.19550536901408766TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCACATCTCGTATG115160.16273507983854238TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG114190.16136435191701246TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATG107090.15133118878004084TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG101980.14411013756455845TruSeq Adapter, Index 9 (97% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT95390.1347976664275665No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATG87180.1231959383494627TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAAGATCTCGTATG81390.11501396446734079TruSeq Adapter, Index 9 (97% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT72080.10185780266379067TruSeq Adapter, Index 9 (100% over 50bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG72020.10177301536967542TruSeq Adapter, Index 9 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)