FastQCFastQC Report
Thu 28 Sept 2023
SRR5413155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5413155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36121521
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG21111035.844446583520113No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA21063865.831387886462477No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA10676942.9558389858500145No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG9102972.5200959837765415No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT8275062.2908946719048737No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA7339562.0319077925871394No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT6431531.7805257979031393No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAG4135881.1449905445565263No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGA3534510.9785053071270172No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGATC2438880.6751875149443458No Hit
CGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGC2188720.6059324024589108No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG1823750.5048929141162135No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG1629780.45119362498605753No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA1578830.4370884603668821No Hit
AAATGGATTTTTGGAGCAGGGAGACTGTAGGCACCATCAATAGATCGGAA1577360.43668150075961637No Hit
CGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCGGAAGAG1448600.40103516128238337No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATCG1438310.3981864440315235No Hit
TCTTTCCCTACACGACGCTCTTCCTGTAGGCACCATCAATAGATCGGAAG1415130.3917692170271567Illumina Single End PCR Primer 1 (95% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCG1381100.38234824054059074No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT1331580.3686389617978711No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG1303680.36091503455793017No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1239650.34318875996390075Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACCA1193390.3303819902821922No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAG1178710.32631793107494006No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGAT1165070.3225417888687467No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA1155580.3199145462340858No Hit
AAATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATAGATC1122510.3107593392869586No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA1086510.3007929815580025No Hit
AGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCA1007230.27884484709267915TruSeq Adapter, Index 5 (97% over 36bp)
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC1003260.2777457793097915No Hit
CGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGGAAGA977680.2706641284568277No Hit
GCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATC905870.2507840132202628No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATC854860.2366622380048725No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCAT827410.22906289023654347No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT801530.22189818640250503No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATA787260.21794763293605493No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA751460.2080366438611486No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATC749770.20756877873442814No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG730650.20227553540727145No Hit
AGGGGGACGGCGGAGCGAGCGCACGGGCTGTAGGCACCATCAATAGATCG729050.2018325861748734No Hit
CCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCTGTAGGCACCATCAATAG696650.19286286421881293No Hit
GCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGG652270.18057655988517204No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATAGATC638000.17662600641872195No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCCTGTAGGCACCATCAATA627580.17374129954272965No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCG623090.17249827325931263No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATC616790.17075416065674534No Hit
GCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCG607440.16816567607991922No Hit
CGGGGGGGATGCGTGCATTTATCAGATCAAAACTGTAGGCACCATCAATA605520.16763413700104157No Hit
AAATGGATTTTTGGAGCAGGGAGCTGTAGGCACCATCAATAGATCGGAAG602010.16666241712246835No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGAT597260.16534741158878663No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC561950.1555720757163022No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAA550300.1523468516179039No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA542550.15020131627347585No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACTGTAGGCACCATCAATAG541420.14988848337809474No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGAT517640.14330515041157874No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGA514640.14247462060083238No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGGCACCATCAATAGATCGG504900.13977816714860927No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATAGAT504390.1396369770807824No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGATCG497740.137795969333628No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATCG493520.13662769073317815No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGGCACCATCAATAGATCG450860.12481755682436518No Hit
CGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATC450350.12467636675653829No Hit
AATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATAGATCG447290.12382922634957703No Hit
ATTAAATGGATTTTTGGAGCAGGGAGACTGTAGGCACCATCAATAGATCG429680.118954016360496No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGAT421000.11655101677473659No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT411800.1140040586884478No Hit
CGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG406230.1124620416731621No Hit
GGTGGCGGCGGCGGCGGGGGCGGCGGGACTGTAGGCACCATCAATAGATC391430.10836476127348016No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGAT385190.10663725926712776No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTCTGTAGGCACCATCAATAGATC379430.10504264203049479No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATC379300.10500665240536244No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA376950.10435607072027782No Hit
CGGGGCCCGAAGCGTTTACTTTGAAACTGTAGGCACCATCAATAGATCGG367230.10166515413345967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)