FastQCFastQC Report
Thu 28 Sept 2023
SRR5413156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5413156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23836959
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA21597719.06059787240478No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG11203044.6998612532748No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT7176533.0106734671985635No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG5342892.2414310483145106No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG4388271.8409521113829999No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA2996561.2571066636478252No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA2418731.0146973865248499No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGA2214630.929074048413642No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG1977750.8296989561462098No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG1842720.7730516296143313No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAG1815980.7618337557236222No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC1688710.7084418780096907No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT1566440.6571475832970136No Hit
CGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGC1313350.5509721269395144No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGATC1161950.48745731366152867No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA1123650.47138982787191935No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAG1053380.44191039637228896No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATCG1037730.43534496157836244No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT950150.39860369772838894No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATA895630.3757316526827101No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCG838280.35167237565832116No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC830900.3485763431484696No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATCG819110.3436302424315115No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGAT746220.3130516774392237No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT737890.30955710415913373No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA693690.2910144704280441No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCG674050.2827751643991165No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA646100.27104967542210395No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGG638380.2678110072681671No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA634560.2662084538552086No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGGCACCATCAATAGATCGG610290.25602678596711936No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATC584280.2451151591945936No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGAT579740.24321055382945453No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA570950.23952300291324916No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATC559910.23489153964647924No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA549690.2306040799919151No Hit
CGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGA542080.22741155866400575No Hit
GCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGG510220.2140457597800122No Hit
TCTTTCCCTACACGACGCTCTTCCTGTAGGCACCATCAATAGATCGGAAG465820.19541922272887244Illumina Single End PCR Primer 1 (95% over 24bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAA452600.18987321327355558No Hit
AAATGGATTTTTGGAGCAGGGAGACTGTAGGCACCATCAATAGATCGGAA451600.18945369667330467No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTCTGTAGGCACCATCAATAGATC450280.1888999347609735No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATAGATC436620.18316933800154625No Hit
CGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCGGAAGAG424160.1779421611624201No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATC424110.17792118533240753No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGATC420580.17644029173352188No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT418520.17557608753700504No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG404260.16959378081742726No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCG400500.16801639840048388No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGA389470.1633891302997165No Hit
CGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG386990.1623487291310943No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT378100.15861922655486382No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT372380.1562195916014287Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT371950.1560391994633208No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA366810.1538828841380312No Hit
AAATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATAGATC357250.14987230543963265No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCAT349400.14657910012766312No Hit
AGGGGGACGGCGGAGCGAGCGCACGGGCTGTAGGCACCATCAATAGATCG340820.1429796476975104No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATC331310.1389900448291244No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACCTGTAGGCACCATCAATAGATCG326170.13683372950383477No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCTGTAGGCACCATCAATAGATCG313620.13156879617068604No Hit
GGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATCG302500.12690377157589608No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCGG301480.12647586464364016No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGCTGTAGGCACCATCAATAGATCGGA300230.12595146889332653No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC295770.12408042485620753No Hit
GGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATAGA288430.12100117301036596No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATAG287330.12053970475008997No Hit
GGTGGCGGCGGCGGCGGGGGCGGCGGGACTGTAGGCACCATCAATAGATC284850.11949930358146775No Hit
CGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATC279870.1174101109122183No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA279630.11730942692815807No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT274420.11512374544085091No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGATCG272080.11414207659626381No Hit
AGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAC269290.1129716252815638TruSeq Adapter, Index 7 (97% over 35bp)
CGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATC267590.11225844706113729No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCCTGTAGGCACCATCAATAGAT267330.11214937274507206No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGCACCATCAAT266990.11200673710098676No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGGCACCATCAATAGATCG262850.11026993837594803No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG260140.10913304838926811No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGAT253290.10625935967754949No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA253060.10616287085949176No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATA250080.1049127113907441No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA242480.10172438522883728No Hit
CGGGGCCCGAAGCGTTTACTTTGAAACTGTAGGCACCATCAATAGATCGG242390.1016866287348147No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACCA239870.10062944690218245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)