FastQCFastQC Report
Thu 28 Sept 2023
SRR5413157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5413157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22312568
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA15665917.021114736770774No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG7310573.2764359530467315No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT6460732.895556441553478No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG5462452.44814940171835No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG4609702.065965692519122No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC3342511.4980391320263988No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG3188021.428800127354234No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC2711731.2153374725849575No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT2340331.0488841983585215No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG2184170.9788967365836151No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAG1851270.8296983117317559No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT1825430.8181173946450271No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATA1787480.8011090431186585No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT1736370.7782026703515257No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA1718690.7702788849763954No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGA1715250.7687371529803293No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA1674490.7504694215385697No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA1667450.7473142490815041No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATCG1451020.6503151049220333No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA1433460.6424451008956029No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAG1347210.6037897565174927No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA1322840.5928676609523387No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC1322280.5926166813250721No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1301890.5834783338251339No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1290200.5782391341059443No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA1271800.5699926606386141No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA1150280.5155300815217684No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT1088290.48774753313917074No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA1082200.48501812969264674No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCG1011420.4532960975177756No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT1004700.45028434199057676No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAA971330.4353286452729242No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT963270.43171633135190896No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA950950.42619477955204443No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA931670.41755390952758104No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGATC900560.40361109487711144No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGAT881610.395118123561573No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT783370.3510891260925233No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC762970.3419462968135268No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG746270.33446172578611305No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCG720940.32310937943135903No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACCA716370.3210612064017015No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA714200.32008866034604355No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA692440.31033630911511395No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTAGGCACCATCAA686910.3078578852958566No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATCG676800.3033268066678833No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATA653220.29275877164833736No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA653190.2927453263111624No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA642580.28799015873027256No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGATC617910.2769336097933685No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA591410.2650568952887897No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGA587360.2632417747701654No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT584190.2618210508086743No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA571140.2559723291375515No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGAT558030.2500967167920788No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA546550.2449516344331141No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATC544240.24391634347063954No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGG535470.2399858232364827No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGCACCATCAAT530320.2376777070214419No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATC525280.23541889037604277No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA517400.23188724847807746No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA517210.23180209467596916No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG513480.2301303910872115No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA511870.2294088246588201No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTAGGCACCATCAAT468340.2098996404179026No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA467170.2093752722680778No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT466800.20920944644291953No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAA466040.2088688312344863No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTCTGTAGGCACCATCAATAGATC451280.20225372534438887No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATC434210.1946033284918168No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGGCACCATCAATAGATCGG425830.19084759764093492No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA423440.18977645244599364No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA416620.18671987912821153No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCGG412420.18483753192371224No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA407090.1824487436856215No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA405280.18163754167606344No Hit
CGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCCTGTAGGCACCATCAATA400630.17955351441393927No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT372290.16685215256262748No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATAGATC367040.1644992185570034No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCCTGTAGGCACCATCAATAGAT355310.1592420917215804No Hit
AGGGGGACGGCGGAGCGAGCGCACGGGCTGTAGGCACCATCAATAGATCG350900.15726562715685616No Hit
CGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGA341220.15292726502839116No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCAA335250.15025164293056723No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT331180.14842755885382625No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCA329320.14759394794897654No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATC323650.14505277922290252No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG316450.14182589830090378No Hit
GCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGG307560.137841596718047No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAAT307550.13783711493898865No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACTGTAGGCACCATCAATAG303800.1361564477921143No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC303030.13581135080462275No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGA301810.1352645737595063No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACCTGTAGGCACCATCAATAGATCG294990.13220800044172415No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATAG293150.13138335309499113No Hit
GGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATCG292710.13118615481642454No Hit
CGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGC289110.12957271435542517No Hit
CGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG286500.1284029700212006No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT272470.12211503400236137No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAG271050.12147862137607827No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAAT263410.11805454217551292No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA252640.11322766612968979No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCCTGTAGGCACCATCAATAGATCGG248240.11125568334402387No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGCTGTAGGCACCATCAATAGATCGGA243860.10929266411647462No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA232690.10428651690831822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)