FastQCFastQC Report
Thu 28 Sept 2023
SRR5413158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5413158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31591231
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA374344211.849623713618504No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG16208045.130550309989503No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT15919455.039199010636844No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG6916452.1893575467192146No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTCTGTAGGCACCATCAATAGATC4273511.3527519709504197No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG4058331.2846381326514311No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGA3924091.242145328239979No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA3425111.0841964341307244No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG3333871.055315001811737No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC3167171.0025471941881592No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT3021480.9564299662776674No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA2658180.8414296992731938No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAG2543720.8051981260242755No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAG2399910.7596759999634076No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC2141720.677947624136584No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG2119260.670838056294799No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTCTGTAGGCACCATCAATAGAT1950340.6173675220190058No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATA1873020.5928923757355324No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATCG1821710.5766505268503149No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTCTGTAGGCACCATCAATAGATCG1705530.539874498717698No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA1663040.5264245638291208No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA1642100.5197961421636277No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACCTGTAGGCACCATCAATAGATCG1639710.5190396031101162No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCG1584550.5015790616073175No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA1582060.5007908682001028No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGATC1577900.49947404708604104No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT1493930.47289388628129114No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATC1472240.4660280569630224No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT1358150.42991360482280666No Hit
GCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGG1256410.39770846536496157No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA1168900.3700077404391111No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA1124400.35592155304109546No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAA1120450.35467120606981095No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGG1086550.34394038016435635No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1076740.3408350880660522No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA1069260.33846734240903753No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1051570.332867687238905No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGATC1019800.32281109906733296No Hit
CGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGC988440.3128842937459449No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGAT944200.29888040766755813No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA937960.2969051759964656No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCG931490.29485713931185525No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT925170.29285658415779997No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT904500.286313629247306No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGAT895530.2834742337201105No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC888410.28122044373642796No Hit
GGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATCG874150.2767065328983223No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG849280.2688340951322853No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGGCACCATCAATAGATCGG827070.26180366317475884No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT826760.2617055346782783No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGA825480.2613003589508747No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA823320.2606166249108811No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT672290.21280905451262727No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACCTGTAGGCACCATCAATAGATC671730.21263179013188818No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGCTGTAGGCACCATCAATAGATC625950.19814042700646897No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA621410.19670331934833435No Hit
CGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGA605210.1915753140483826No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA604860.1914645238104207No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATAGATC600420.19005907050598944No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA593750.1879477251139723No Hit
GGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATCG588430.1862637134969511No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGCACCATCAAT587870.18608644911621203No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACCTGTAGGCACCATCAATAGATCGG565460.1789927084512788No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACTGTAGGCACCATCAATAG540640.17113609786209344No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAAT516800.16358969993920147No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCCTGTAGGCACCATCAATAGATC500420.15840471680258358No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA485860.1537958429033677No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATC485350.15363440569948034No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATA471780.14933890990192816No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTAGGCACCATCAA469470.1486076943313795No Hit
AGGGGGACGGCGGAGCGAGCGCACGGGCTGTAGGCACCATCAATAGATCG459680.14550873310381604No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCTGTAGGCACCATCAATAGATCG436850.1382820441533285No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCCTGTAGGCACCATCAATAGAT434430.13751600879370607No Hit
GGTGGCGGCGGCGGCGGGGGCGGCGGGACTGTAGGCACCATCAATAGATC420680.13316353515948776No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA420190.1330084288263411No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATC411200.13016270242840491No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCAT409860.12973853408877925No Hit
AAATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATAGATC408240.12922573355878408No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA407930.12912760506230353No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA375800.11895706121739923No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG374110.11842210263981166Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG372370.1178713168853724No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA365160.11558903798335683No Hit
CCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCGG349090.11050218334321951No Hit
GCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCG346650.1097298171128564No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTAGGCACCATCAAT345950.10950823663693257No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA343990.10888781130434583No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA340800.10787803742120716No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGAT337170.10672898438177354No Hit
CACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATC336790.10660869783770059No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA335130.10608323556622407No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGAT332370.10520957540401006No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGA328960.10413016194272393No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGGCACCATCAATAGATCG316380.10014804424683546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)