FastQCFastQC Report
Tue 4 Jul 2023
SRR5443325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125024824
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5131601541.04466085871075No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC45761223.6601707193764974No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA34785662.782300257427277No Hit
NGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC18305531.4641516311992568No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT18243471.4591878169730517Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC6753180.5401471311009404No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC6417520.5132996627933665No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT6259230.5006389771042589Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC5856340.46841417669182245No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4414830.3531162739329271No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4322330.34571774322193805No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4203580.33621962947134404No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC3924310.3138824654534207No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3906860.31248674263280707No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3613700.28903859924649844No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC3608180.28859708692731295No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3604660.2883155428397164No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3174810.2539343706654608No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3083100.24659902740594938No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA3078830.246257495231507No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC3062250.24493135859163456No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCCTAGATCGGAAGAGCACACGTC2903960.23227067290252693No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC2537200.20293569859374488No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATAGGAAGAGCACACGTC2153340.17223299590487726No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGA2102190.1681418083819898No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAAACGTC1868470.14944792083850483No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCCTAGATCGGAAGAGCACACGTC1837810.1469956078482462No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1803270.144232956488705No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCAGATCGGAAGAGCACACGTCTG1798250.14383143622741673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1780700.14242771499522366No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAGGAGCACACGTC1768620.1414615068764264No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTT1752860.14020095721150547No Hit
TGGATGACCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1698240.13583222480681117No Hit
TGGATGATCCTATGCCGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1661970.13293120092694552No Hit
NGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC1638460.13105077436461737No Hit
TGGATGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1620330.12960066234526355No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGACGAGCACACGTC1575560.12601977348114482No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTA1560390.12480641444454263No Hit
TGGATGATCCTATGCTGCCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1494890.11956745486000443No Hit
TGGATGATCCTATGCTGTCGGGAAAAGCCTAGATCGGAAGAGCACACGTC1466070.11726231264280763No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1412100.11294556991337977No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1351620.10810813059012984No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT1322970.10581658567261812No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGAG1293580.10346585250941845No Hit
TGGATGATCCCATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1290130.10318990730992751No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTG1287950.10301554193749554No Hit
TGGATGATCCTATGCTGTCGAGGAAAGCCTAGATCGGAAGAGCACACGTC1255130.10039046325712084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)