FastQCFastQC Report
Tue 4 Jul 2023
SRR5443326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124524054
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5153614041.386493889766875No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC44866523.603040421411272No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA26500112.128111730124045No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT25370472.0373951204640353Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC9565540.7681680520937746No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC8410640.675422918691677No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT6449130.5179023484089267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC6231800.5004494954846234No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5708640.4584367290194391No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5707590.4583524079612763No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5066210.406845893404659No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4794210.38500272405201325No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC4649540.37338488835257483No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC3871970.31094153102339567No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3666880.29447162071996147No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCCTAGATCGGAAGAGCACACGTC3590620.28834750272425275No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3526120.2831677805799673No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3346650.2687553040957051No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3316390.26632525150522324No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3172700.2547861154600701No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC2892200.2322603470651542No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCAGATCGGAAGAGCACACGTCTG2670940.21449189246601302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC2448230.19660699450083757No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT2396860.19248168711243532No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA2310630.18555692059302856No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGA2135060.17145763661051383No Hit
TGGATGATCCTATGCCGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2092810.16806471784158264No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCCTAGATCGGAAGAGCACACGTC2042960.16406147522309225No Hit
TGGATGACCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1899540.15254402173575235No Hit
TGGATGATCCCATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1685790.13537866346689933No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1681210.13501086304177023No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGAG1667540.1339130831702604No Hit
TGGATGATCCTATGCTGTCGGGAAAAGCCTAGATCGGAAGAGCACACGTC1656120.13299599128052803No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1558290.12513967783284666No Hit
GAAGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1469080.11797560011979694No Hit
TGGATGATCCTATGCTGCCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1455060.11684971322889955No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1453670.11673808820904595No Hit
TGGATGATCCTATGCTGTCGAGGAAAGCCTAGATCGGAAGAGCACACGTC1420430.11406872442492114No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTT1334850.10719615665580563No Hit
TGGATGATCCTACGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1333470.10708533469364884No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1257780.1010069909866571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)