FastQCFastQC Report
Tue 4 Jul 2023
SRR5443327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132160059
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3523126526.658027596673517No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1522060111.516793436056199No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC32909502.4901244936641564No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC15394831.1648625247662758No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA14272231.0799200687402841No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA8612700.6516870577365587No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC7892340.5971804234742359No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA7660670.5796509216146764No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA6040640.4570700138685623No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT5957750.45079807356926194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC5039590.3813247389667101No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC4776130.36138982050545243No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4641900.35123319671036163No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA4419840.3344308434365938No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT4140140.31326711196459134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT3783640.286292245072318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3660960.27700956156504136No Hit
TGGATGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3485560.26373777572239127No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3375830.2554349646590276No Hit
GAAGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3373940.2552919562482943No Hit
TACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3044120.23033585358795883No Hit
TCCGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2995420.22665092787224014No Hit
TCCTTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2921360.22104711681461947No Hit
GAAGTGGATGCTCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2640160.19976988660393985No Hit
GAAGTGGATGCTCCTCTGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2571760.19459434412026103No Hit
GAATTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2544250.19251277725292176No Hit
TAATTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2494600.18875596900270755No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC2430040.18387098329004226No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2329430.17625824455783573No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2309960.1747850309298061No Hit
TACTTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2302380.1742114839703575No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2262700.1712090640032175No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCCTAGATCGGAAGAGCACACGTC2195210.1661023774210028No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACACG2193300.1659578556937539No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGT2165600.16386191231951552No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTC2147630.16250219743016306No Hit
TCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1795280.1358413437148965No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1792170.13560602299670582No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGAGATCGGAAGAGCACACGTC1764410.1335055396729204No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC1700640.1286803299626251No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1659800.12559013763757473No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1553040.11751205407679186No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACAC1546510.11701795623441724No Hit
AAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1461950.1106196540060564No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACAGATCGGAAGAGCACA1449880.10970636748883413No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCCTAGATCGGAAGAGCACACGTC1418430.10732667726790286No Hit
TCATTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1401220.10602446840614682No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGAGATCGGAAGAGCACACGT1372730.10386874902953849No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGT1336280.10111072967968332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)