FastQCFastQC Report
Tue 4 Jul 2023
SRR5443328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113065267
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4601618840.698783296553835No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC39086803.4570121344161335No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA25421082.2483544836098956No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC7350560.650116538441465No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT7032290.6219673102616031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC6709190.5933908951897668No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4737710.4190243498916427No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4582730.40531722266219916No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3785100.3347712432324597No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC3738580.3306568055068583No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3728980.32980773839237476No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3541830.3132553518845005No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC3327740.29432027078660683No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC3032910.2682441814779423No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3029200.26791605241599087No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT2936280.259697790303719Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2762220.24430314218423949No Hit
NGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2746920.24294994147053134No Hit
TGGATGATCCTATGCCGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2429730.21489623334104893No Hit
TGGATGATCCCATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2378910.21040148430375175No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2373240.2099000040392599No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2367560.20939763932985717No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCCTAGATCGGAAGAGCACACGTC2289630.2025051601390549No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC2262080.20006851440946935No Hit
GAAGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2218080.19617695680141986No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2160220.1910595585468347No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2090480.18489143973807623No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA2061180.18230001614907962No Hit
TGGATGACCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1897040.1677827373812331No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCCTAGATCGGAAGAGCACACGTC1726970.1527409827812108No Hit
TGGATGATCCTATGCTGTCGAGAAATGCCTAGATCGGAAGAGCACACGTC1568730.13874552651080727No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1515430.1340314351356018No Hit
TCCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1459000.1290405125032783No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1368740.12105751273731127No Hit
TGGATGATCCTATGCTGTCGAGATAAGCCTAGATCGGAAGAGCACACGTC1335580.1181246934126994No Hit
TGGATGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1317580.11653269257304279No Hit
TGGATGATCCTATGCTGCCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1283230.1134946243040314No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1277700.11300552626829245No Hit
TGGATGATCCTATGCTGTTGAGAAAAGCCTAGATCGGAAGAGCACACGTC1273480.11263229051588405No Hit
TGGATGATCCTATGCTGTCGGGAAAAGCCTAGATCGGAAGAGCACACGTC1211190.10712308316576125No Hit
GAATTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1207730.1068170652265828No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCTCACGTC1202120.10632089163155649No Hit
TGGATGATCCTATGCTGTCGAGGAAAGCCTAGATCGGAAGAGCACACGTC1163100.10286978758914529No Hit
TGGATGATCCTACGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1158420.10245586737083459No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)