FastQCFastQC Report
Tue 4 Jul 2023
SRR5443329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162942510
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2484698215.248925525941633No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC156731799.618839798159486No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC65252024.004603832357805No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT59167613.6311954443318686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGT31290801.9203582907861183No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC29450811.8074356409509096No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA26931931.6528486028599902No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC19811111.21583434550014No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTC19340411.1869468562869199No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC16612871.019554074624234No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACACG14431570.8856847731141492No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGAGATCGGAAGAGCACACGTC13016090.7988148703490575No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTGACTAGATCGGAAGAGCACA10715490.6576239681099794No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGTAGATCGGAAGAGCACACGT10502880.6445758077496166No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTCT10218280.6271095247029151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAAGATCGGAAGAGCA9288080.5700219052719883No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGAGATCGGAAGAGCACACG9168050.5626555034656088No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCAGATCGGAAGAGCACACGTCT8237570.5055507000597941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACGTC7444420.4568740226230712No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACAC6464770.39675159048427566No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGT6140860.37687280010600055No Hit
GGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCTG6100050.37436823576609934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC5926170.36369698736075684No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT5773100.3543028765176135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5735310.35198365362114525No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGAGATCGGAAGAGCACACGT5271300.3235067386650666No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACGT4958190.3042907587467506No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGTAGATCGGAAGAGCACACGTC4948660.3037058898871755No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGTAGATCGGAAGAGCACACGTCT4516910.2772088143235304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGTCT4407440.2704904938557777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGTC4170600.2559553059542289No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGGACAGATCGGAAGAGCACACGTC4017840.24658022022613987No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG3348900.20552647679233615TruSeq Adapter, Index 6 (100% over 49bp)
GCCCTCATCGTCTAGTGGCCCAGGACGCCGTAGATCGGAAGAGCACACGT3204780.19668163943221445No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGCAGATCGGAAGAGCAC3199190.1963385736478467No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACG3100760.1902977927613856No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGA2773760.17022936494595547No Hit
CGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACACGT2716990.16674531403744794No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACA2592000.15907451039019835No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGAGATCGGAAGAGCACA2580860.15839083367501827No Hit
CCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTCTG2454570.15064024728721803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2395790.14703283998755143No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGAGATCGGAAGAGCACACGTC2312610.14192797201908822No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC2150700.13199133854020045No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCTAGATCGGAAGAGCACACGT2083450.127864116000177No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCTAGATCGGAAGAGCACACGTC1973070.12108994761403885No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAGGCCTAGATCGGAAGAGCACA1950980.11973425473806683No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGTC1941900.11917700298099004No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACAC1909530.11719041274127912No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACCAGATCGGAAGAGCAC1803700.1106954839470682No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT1802510.11062245205379492No Hit
GCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACACG1800170.11047884312080378No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGATCGGAAGAGCACACGTCTGAA1685080.10341561572851676Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)