FastQCFastQC Report
Mon 3 Jul 2023
SRR5443330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166664595
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2257758513.546719385721964No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC118337597.10034365727166No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC96343785.78069865408427No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT76705674.602397407799779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC33926412.03560990263109No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGT27173431.6304260661960028No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA19483561.1690281310196686No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTC17956821.0774225923628231No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTGACTAGATCGGAAGAGCACA16508370.9905145120953853No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC16464460.9878798793469004No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGTAGATCGGAAGAGCACACGT16233310.9740107069530873No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGGACAGATCGGAAGAGCACACGTC16207570.9724662877559569No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACACG15148510.908921897899191No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGAGATCGGAAGAGCACACGTC12313680.7388299836566968No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAAGATCGGAAGAGCA12282220.7369423601935372No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACAC11389240.6833628942007749No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGAGATCGGAAGAGCACACG11058560.6635218475765653No Hit
ACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATCGGAAGAG11050460.6630358415355103No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC10054070.6032516984186114No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGT9379210.5627595951017671No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTCT9108310.5465053930620358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCTG6659190.3995563664856355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACGTC6233910.3740392493078689No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCAGATCGGAAGAGCACACGTCT6214440.3728710347869624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCAGATCGGAAGA5762940.34578069805407685No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGTAGATCGGAAGAGCACACGTC5601810.3361127778818291No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGAGATCGGAAGAGCACACGT5339010.32034458188315285No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACGT4979700.29878571390642383No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT4670950.28026048363781164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTGTAGTTTAATGGTAGAACATGAGTAGATCGGAAGAGCACACGTCT4505820.2703525604823268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGTGTTGGGGACTCACGGGAGACCGCCGGGGTCAGATCGGAAGAGCA4230460.2538307551162861No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC4180670.2508433179824425No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACG3982810.2389715704166203No Hit
AGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAGATCGGAAGAG3787990.22728222511805823No Hit
CCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGTCT3635370.21812491129264738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACACGT3600050.21600568495066397No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGAGATCGGAAGAGCACA3399220.20395573516978818No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGAGATCGGAAGAGCACACGTC3357530.20145430407699969No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCTAGATCGGAAGAGCACACGTC3212770.1927685961136497No Hit
CCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGTC3093040.18558470681790576No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGCAGATCGGAAGAGCAC3061680.18370308342932704No Hit
AGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGATCGGAAGAGCAC2945560.17673579682595456No Hit
ATCCTTGAAGGTTGAAAGCTCCGGCGGTGCAGATCGGAAGAGCACACGTC2877780.17266894627500218No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGATCGGAAGAGCACACGTCTGAA2763500.16581206104391877Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGTGTAGTTTAATGGTAGAACATGAGCTAGATCGGAAGAGCACACGT2747220.16483524890214388No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGTC2705050.1623050174513669No Hit
GGGGTGTTGGGGACTCACGGGAGACCGCCGGGGTCAGATCGGAAGAGCAC2498300.14989986325530028No Hit
GCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACACG2417910.145076403299693No Hit
ATGGGCTGTCACCCGCTGCTGAACACATAGGCAGATCGGAAGAGCACACG2407150.14443079527478528No Hit
GGAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2138230.12829539471175627No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGTAGATCGGAAGAGCACACGT2095820.12575076308198513No Hit
TGGCTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2018570.12111570546821898No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT2002930.12017729380376198No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC1974280.11845827243632638No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACA1948130.11688925293341396No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT1869070.11214559396973305No Hit
CCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTCTG1786160.10717093213468644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGA1765420.10592651666660217No Hit
GGGTCGTAGCTCAATTGGTAGAGCACTGGTAGATCGGAAGAGCACACGTC1752120.10512850674733887No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACAC1743600.1046173003930439No Hit
AACCCGGATTACGGGTCAACGTTAGAACATCAAACAGATCGGAAGAGCAC1743100.10458730002013925No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACA1711110.1026678761617007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)