FastQCFastQC Report
Fri 7 Jul 2023
SRR5443331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146033026
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4483114530.69931934437899No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA91830586.2883432957144905No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC54221803.712982020929978No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA11375330.7789559876681594No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT11190820.7663211744992533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC9292720.6363437267950608No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT7342430.5027924299808729Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC6676240.45717329722387595No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGAACAGATCGGAAGAGCACACGTC6342870.43434489948869515No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGT5231040.3582093820338969No Hit
GGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCT5113320.3501481918206639Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC4739910.3245779485525418No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC4595280.314674024490871No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT4523580.3097641762213432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC4301120.29453063583028133No Hit
NGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4159220.2848136557822201No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3728410.2553127947920493No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTC3720000.25473689766587454No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGGTAGATCGGAAGAGCACACGT3664490.25093570272247867No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC3568210.2443426735538576No Hit
GGGGTGTTGGGGACTCACGGGAGACCGCCGGGGTAGATCGGAAGAGCACA3518840.24096193144693173No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCCTAGATCGGAAGAGCACACGTC3480540.23833923704354382No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3455160.23660127401592018TruSeq Adapter, Index 5 (100% over 49bp)
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2658660.18205881729794465No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC2577990.17653472441227097No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGAG2516160.17230075065348574No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATCCAGATCGGAAGA2458090.1683242528987929No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2406690.1648045011407214No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2371860.16241942421983369No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2243570.15363442513339412No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT2167130.1483999927523244No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCCTAGATCGGAAGAGCACACGTC2096850.14358738276093794No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA2079140.14237464339059852No Hit
CCCTGTGGGAGAGTAGGACGCCGCCGAACAGATCGGAAGAGCACACGTCT1893420.12965697225229036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1845970.1264077072538372No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGGTACAAAAGATCGGAAGAGCA1819700.12460879910822363No Hit
TGGATGATCCTATGCTGTCGGGAAAAGCCTAGATCGGAAGAGCACACGTC1736920.11894021835855131No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1689670.11570464889223073No Hit
ATGTCGGCTCGTCGCATCCTGGGGCTGAGATCGGAAGAGCACACGTCTGA1661110.11374892690369917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1634140.11190208439562158No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1631780.11174047711645721No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA1599920.10955877884773818No Hit
TGGATGACCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1597220.10937388916394844No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1563550.10706824632942963No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGAAAGATCGGAAGAGCACACGTCT1559390.10678337926107208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCA1555910.1065450770019653No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA1548800.1060582008346523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGATGATCCTATGCCGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1519460.1040490662708037No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTT1498610.10262130704598288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)